Development of electrochemical sensor for detection of mercury by exploiting His-Phe-His-Ala-His-Phe-Ala-Phe modified electrode

Daud Nurulhaidah, Nor Azah Yusof, Tan Wee Tee

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

A sensitive voltammetric method for detection of mercury ions is described which is made by modifying a gold electrode with 3-mercaptopropionic acid followed by covalent attachment of the octapeptide His-Phe-His-Ala-His-Phe-Ala-Phe to the self-assembled monolayer using carbodiimide coupling. A linear working range for concentration of mercury between 0.25 to 0.81 with LOD 9.5×10-9 M was obtained which is below the WHO guidelines for drinking water. The reproducibility of the analytical signal is 4.5% in indicating a reproducible and reliable detection system. The developed method was applied for the detection of Hg(II) in spiked wastewater and validated against ICPMS. Good agreement was obtained between the developed method and ICPMS. Insignificant interference was observed by As3+, Cr3+, Cu2+, Ni2+, Pb2+and Zn2+ in detection of Hg(II) thus making the developed system highly potential for electrochemical sensor in Hg(II) detection.

Original languageEnglish
Pages (from-to)2798-2807
Number of pages10
JournalInternational Journal of Electrochemical Science
Volume6
Issue number7
Publication statusPublished - Jul 2011
Externally publishedYes

Fingerprint

Electrochemical sensors
Self assembled monolayers
Mercury
Potable water
Wastewater
3-Mercaptopropionic Acid
Gold
Carbodiimides
Electrodes
Acids
Ions
Drinking Water

Keywords

  • Cyclic voltammetry
  • Hg(II) detection
  • Peptide modified electrode

ASJC Scopus subject areas

  • Electrochemistry

Cite this

Development of electrochemical sensor for detection of mercury by exploiting His-Phe-His-Ala-His-Phe-Ala-Phe modified electrode. / Nurulhaidah, Daud; Yusof, Nor Azah; Tee, Tan Wee.

In: International Journal of Electrochemical Science, Vol. 6, No. 7, 07.2011, p. 2798-2807.

Research output: Contribution to journalArticle

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