Development of electrochemical biosensor for formaldehyde determination based on immobilized enzyme

Nur Indang Marzuki, Fatimah Abu Bakar, Abu Bakar Salleh, Yook Heng Lee, Nor Azah Yusof, Shafiquzzaman Siddiquee

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The newly formaldehyde biosensor developed that is based on coupled with enzyme (nicotinamide adenine dinucleotide, NAD +) and formaldehyde dehydrogenase (FDH) for the detection of formaldehyde in fish samples. To maximize the reaction rate, the enzyme acts as biorecognition immobilized with Nafion membrane which chemically modified on gold electrode. The enzyme required nicotinamide adenide dinucleotide (NAD +) as a cofactor which then reduced to NADH during enzymatic reaction. In the system, 0.1 M of potassium phosphate was used as the supporting electrolyte and 0.5 mM of NAD + was added as the coenzyme. The optimum scan rate was found at 0.1 V/s while the optimum pH was at 8 via cyclic voltammetry. A linear response was ranged from 1 to 10 ppm of formaldehyde, with correlation coefficient (R 2) equals to 0.9865 (RSD < 3.05%). The response time was found less than 1 min. Formaldehyde biosensor showed reproducibility with no significant different (p > 0.05) at 1, 5 and 10 ppm of formaldehyde (n = 10). For interferences study, it was showed that the biosensor response retained its specificity for formaldehyde and did not respond to equivalent additions of methanol and also ethanol and gave the percentage of formaldehyde recovered ranging from 99.0% to 99.8%. Thus, formaldehyde biosensor is a promising tool and has a potential application for simple, fast, reusability, reproducibility, sensitivity, storage stability, validity, interferences and convenient method for the determination of formaldehyde in fish samples.

Original languageEnglish
Pages (from-to)6070-6081
Number of pages12
JournalInternational Journal of Electrochemical Science
Volume7
Issue number7
Publication statusPublished - Jul 2012

Fingerprint

Immobilized Enzymes
Formaldehyde
Biosensors
Enzymes
NAD
glutathione-independent formaldehyde dehydrogenase
Fish
Coenzymes
Niacinamide
Reusability
Gold
Electrolytes
Cyclic voltammetry
Reaction rates
Methanol
Ethanol
Potassium
Phosphates
Membranes
Electrodes

Keywords

  • Formaldehyde
  • Formaldehyde dehydrogenase
  • Voltammograms

ASJC Scopus subject areas

  • Electrochemistry

Cite this

Development of electrochemical biosensor for formaldehyde determination based on immobilized enzyme. / Marzuki, Nur Indang; Bakar, Fatimah Abu; Salleh, Abu Bakar; Lee, Yook Heng; Yusof, Nor Azah; Siddiquee, Shafiquzzaman.

In: International Journal of Electrochemical Science, Vol. 7, No. 7, 07.2012, p. 6070-6081.

Research output: Contribution to journalArticle

Marzuki, Nur Indang ; Bakar, Fatimah Abu ; Salleh, Abu Bakar ; Lee, Yook Heng ; Yusof, Nor Azah ; Siddiquee, Shafiquzzaman. / Development of electrochemical biosensor for formaldehyde determination based on immobilized enzyme. In: International Journal of Electrochemical Science. 2012 ; Vol. 7, No. 7. pp. 6070-6081.
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