Development of an electrochemical biosensor methods based on acrylic microsphere for the determination of Arowana DNA hybridization

Mahbubur Rahman, Yook Heng Lee, Dedi Futra, Chew Poh Chiang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

An electrochemical method of Arowana DNA determination based of N-acrylosuccinimide (NAS) modified acrylic microsphere was fabricated. Hydrophobic succinimide functional group containing poly(n-butylacrylate-N-acryloxysuccinimide) microspheres were synthesized with a simple one-step photopolymerization pocedure. Aminated DNA probe was covalently bonded to the succinimde functional group of the acrylic microspheres. The hybridization of the immobilized DNA probe with the complementary DNA was determined by the differential pulse voltametry using anthraquninone-2-sulfonic acid monohydrate sodium salt (AQMS) as the electroactive hybridization label. The influences of many factors such as duration of DNA probe immobilization and hybridization, operational temperature and non-complementary DNA on the biosensor performance were evaluated. Under optimized conditions, the DNA microbiosensor demonstrated a wide linear response range to target DNA is 1.0 × 10-16 and 1.0 × 10-8 M with a lower limit of detection (LOD) of 9.46 × 10-17 M (R2 = 0.99) were calculated. This biosensor had improved the overall analytical performance of the resultant DNA microbiosensor when compared with other reported DNA biosensors using other nano-materials for membranes and microspheres as DNA immobilization matrices.

Original languageEnglish
Title of host publication2015 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium
PublisherAmerican Institute of Physics Inc.
Volume1678
ISBN (Electronic)9780735413252
DOIs
Publication statusPublished - 25 Sep 2015
Event2015 Postgraduate Colloquium of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology, UKM FST 2015 - Selangor, Malaysia
Duration: 15 Apr 201516 Apr 2015

Other

Other2015 Postgraduate Colloquium of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology, UKM FST 2015
CountryMalaysia
CitySelangor
Period15/4/1516/4/15

Fingerprint

bioinstrumentation
deoxyribonucleic acid
immobilization
probes
complementary DNA
succinimides
sulfonic acid
sodium
membranes
salts
matrices
pulses

Keywords

  • acrylic microspheres
  • Arowana DNA
  • DNA hybridization
  • DNA microbiosensor
  • photopolymerization

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

Rahman, M., Lee, Y. H., Futra, D., & Chiang, C. P. (2015). Development of an electrochemical biosensor methods based on acrylic microsphere for the determination of Arowana DNA hybridization. In 2015 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium (Vol. 1678). [050012] American Institute of Physics Inc.. https://doi.org/10.1063/1.4931291

Development of an electrochemical biosensor methods based on acrylic microsphere for the determination of Arowana DNA hybridization. / Rahman, Mahbubur; Lee, Yook Heng; Futra, Dedi; Chiang, Chew Poh.

2015 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium. Vol. 1678 American Institute of Physics Inc., 2015. 050012.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Rahman, M, Lee, YH, Futra, D & Chiang, CP 2015, Development of an electrochemical biosensor methods based on acrylic microsphere for the determination of Arowana DNA hybridization. in 2015 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium. vol. 1678, 050012, American Institute of Physics Inc., 2015 Postgraduate Colloquium of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology, UKM FST 2015, Selangor, Malaysia, 15/4/15. https://doi.org/10.1063/1.4931291
Rahman M, Lee YH, Futra D, Chiang CP. Development of an electrochemical biosensor methods based on acrylic microsphere for the determination of Arowana DNA hybridization. In 2015 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium. Vol. 1678. American Institute of Physics Inc. 2015. 050012 https://doi.org/10.1063/1.4931291
Rahman, Mahbubur ; Lee, Yook Heng ; Futra, Dedi ; Chiang, Chew Poh. / Development of an electrochemical biosensor methods based on acrylic microsphere for the determination of Arowana DNA hybridization. 2015 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium. Vol. 1678 American Institute of Physics Inc., 2015.
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