Development of a rapid continuous flow salt leaching kit for fabrication of poly(3-hydroxybutyric acid-co-3 hydroxyvalerate) (PHBV) porous 3-D scaffold

Syazwan Aizad, Badrul Hisham Yahaya, Saiful Irwan Zubairi

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Polyhydroxyalkanoates (PHAs) that are synthesized from bacteria that are predominantly produced by microbial fermentation processes on organic waste, such as palm oil mill effluent (POME), olive oil and kitchen waste, contribute to a sustainable waste management. A great variety of materials from this family can be produced, however the application of PHAs in the production of scaffolds in tissue engineering has been mainly constrained to poly(hydroxybutyrate-co-valerate) (PHBV) due to its highly adjustable physico-chemical properties. One of the common methods in making the 3-D scaffolds is by performing solvent-casting particulate-leaching (SCPL) process, but this process requires a long period of soaking in water to extract the entire salt particle in the 3-D scaffolds. Therefore, the objective of this study is to develop a new method to the conventional method of salt leaching process via a highly efficient continuous flow leaching kit. The salt leaching process was carried out by (1) immersing the 3-D porous scaffolds in a fabricated static container containing tap water and (2) by allowing a presetting continuous flow rate of water. The concentration of sodium chloride (NaCl) was calculated periodically for both processes based on the salt standard calibration curve. The results showed that the exhaustive salt leaching of the conventional process occurred at 48 ± 5 hrs with the needs of changing the water twice a day. In contrast, the exhaustive salt leaching process via continuous flow leaching kit occurred at 40 ± 5 mins, 72 times faster than the conventional method (p<0.05). Therefore, the salt leaching process using continuous flow leaching kit can be considered a highly efficient and time saving procedure as compared to the conventional method.

Original languageEnglish
Pages (from-to)193-199
Number of pages7
JournalJurnal Teknologi
Volume75
Issue number1
Publication statusPublished - 1 Jul 2015

Fingerprint

Scaffolds
Leaching
Salts
Fabrication
Acids
Water
Olive oil
Kitchens
Palm oil
Sodium chloride
Scaffolds (biology)
Waste management
Tissue engineering
Fermentation
Chemical properties
Containers
Effluents
Bacteria
Casting
Flow rate

Keywords

  • 3-D scaffold
  • Continuous flow
  • PHBV
  • Salt leaching
  • Salt leaching kit

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Development of a rapid continuous flow salt leaching kit for fabrication of poly(3-hydroxybutyric acid-co-3 hydroxyvalerate) (PHBV) porous 3-D scaffold. / Aizad, Syazwan; Yahaya, Badrul Hisham; Zubairi, Saiful Irwan.

In: Jurnal Teknologi, Vol. 75, No. 1, 01.07.2015, p. 193-199.

Research output: Contribution to journalArticle

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