Development of a portable low-cost real-time PCR system

K. S. Chong, Gan Kok Beng, Sue Mian Then

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Real-time PCR instrument monitors the fluorescence emitted during PCR reaction as an indicator of amplicon production during each PCR cycle. It has been used for specific detection and quantification of nucleic acid targets. However, the lack of affordable and portable real-time PCR system tends to limit laboratory infrastructure and near field testing. In this work, we proposed a portable affordable real-time PCR system designed by using commercial available off-the-shelf items. The dimension and the weight of the system are equal to 220 mm × 150 mm × 400 mm and 3.6 kg respectively. The prototyping cost of proposed system is equal to USD 2665. The temperature ramp rate of proposed system is equal to 3.0 °C/s. It is successfully amplified DNA sample and recorded fluorescence signal.

Original languageEnglish
Title of host publicationProceeding of 2017 International Conference on Robotics, Automation and Sciences, ICORAS 2017
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages1-5
Number of pages5
Volume2018-March
ISBN (Electronic)9781538619087
DOIs
Publication statusPublished - 7 Mar 2018
Event2017 International Conference on Robotics, Automation and Sciences, ICORAS 2017 - Melaka, Malaysia
Duration: 27 Nov 201729 Nov 2017

Other

Other2017 International Conference on Robotics, Automation and Sciences, ICORAS 2017
CountryMalaysia
CityMelaka
Period27/11/1729/11/17

Fingerprint

Computer Systems
Real time systems
Real-Time Polymerase Chain Reaction
Fluorescence
Real-time
Costs and Cost Analysis
Nucleic acids
Costs
Polymerase Chain Reaction
Architectural Accessibility
DNA
Nucleic Acids
Testing
Prototyping
Weights and Measures
Near-field
Temperature
Quantification
Monitor
Infrastructure

Keywords

  • Fluorescence detection
  • PCR
  • real-time PCR

ASJC Scopus subject areas

  • Artificial Intelligence
  • Computer Networks and Communications
  • Computer Science Applications
  • Computer Vision and Pattern Recognition
  • Signal Processing
  • Information Systems and Management
  • Control and Optimization
  • Health Informatics

Cite this

Chong, K. S., Kok Beng, G., & Then, S. M. (2018). Development of a portable low-cost real-time PCR system. In Proceeding of 2017 International Conference on Robotics, Automation and Sciences, ICORAS 2017 (Vol. 2018-March, pp. 1-5). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/ICORAS.2017.8308041

Development of a portable low-cost real-time PCR system. / Chong, K. S.; Kok Beng, Gan; Then, Sue Mian.

Proceeding of 2017 International Conference on Robotics, Automation and Sciences, ICORAS 2017. Vol. 2018-March Institute of Electrical and Electronics Engineers Inc., 2018. p. 1-5.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Chong, KS, Kok Beng, G & Then, SM 2018, Development of a portable low-cost real-time PCR system. in Proceeding of 2017 International Conference on Robotics, Automation and Sciences, ICORAS 2017. vol. 2018-March, Institute of Electrical and Electronics Engineers Inc., pp. 1-5, 2017 International Conference on Robotics, Automation and Sciences, ICORAS 2017, Melaka, Malaysia, 27/11/17. https://doi.org/10.1109/ICORAS.2017.8308041
Chong KS, Kok Beng G, Then SM. Development of a portable low-cost real-time PCR system. In Proceeding of 2017 International Conference on Robotics, Automation and Sciences, ICORAS 2017. Vol. 2018-March. Institute of Electrical and Electronics Engineers Inc. 2018. p. 1-5 https://doi.org/10.1109/ICORAS.2017.8308041
Chong, K. S. ; Kok Beng, Gan ; Then, Sue Mian. / Development of a portable low-cost real-time PCR system. Proceeding of 2017 International Conference on Robotics, Automation and Sciences, ICORAS 2017. Vol. 2018-March Institute of Electrical and Electronics Engineers Inc., 2018. pp. 1-5
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