Development of a Polygonum minus cell suspension culture system and analysis of secondary metabolites enhanced by elicitation

Muhammad Faizan A Shukor, Ismanizan Ismail, Zamri Zainal, Normah Mohd. Noor

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14 Citations (Scopus)

Abstract

Polygonum minus has been reported to contain valuable metabolites and to date, there is no report on using cell culture technique for metabolite production in P. minus. Naphthalene acetic acid (NAA) concentrations in the range of 2-6 mg L-1 were used in a matrix of combinations with dichlorophenoxyacetic acid (2,4-D) concentrations in the range of 2-10 mg L-1 as plant growth regulators (PGRs) to induce callus cultures. Media that were supplemented with 2 mg L-1 2,4-D + 4 mg L-1 NAA, 2 mg L-1 2,4-D + 6 mg L-1 NAA and 6 mg L-1 2,4-D + 8 mg L-1 NAA were effective for callus induction (93. 3 % of the explants produced callus). To establish cell culture, the best growth was obtained from medium that was supplemented with 1 mg L-1 2,4-D + 2 mg L-1 NAA. From a 1-g inoculum size, the fresh weight increases exponentially after 5-10 days of culture, and a 26. 71 g maximum fresh weight was obtained after 25 days of culture. The cell culture medium was then analyzed using gas chromatography-mass spectrometry (GC-MS). Jasmonic acid (100, 50, 25 and 5 μM), salicylic acid (100, 50, 25 and 5 μM), yeast extract (500, 250 and 100 mg L-1) and glass beads were used in this research as elicitors. The cell cultures were then incubated with the different elicitors for 1, 2, 3 and 4 days. Several compounds with high peak area percentages were detected, including 2-furancarboxaldehyde, 5-hydroxymethyl, furfural, and 2-cyclopenten-1-one, 2-hydroxy. These results show the diversity of metabolites released by P. minus cell into the culture medium under control conditions.

Original languageEnglish
Pages (from-to)1675-1689
Number of pages15
JournalActa Physiologiae Plantarum
Volume35
Issue number5
DOIs
Publication statusPublished - 2013

Fingerprint

Polygonum
2,4-Dichlorophenoxyacetic Acid
Systems Analysis
naphthaleneacetic acid
Acetic Acid
cell suspension culture
secondary metabolites
2,4-D
Suspensions
cell culture
Cell Culture Techniques
Bony Callus
metabolites
Culture Media
callus
culture media
Weights and Measures
hydroxymethylfurfural
Plant Growth Regulators
Salicylic Acid

Keywords

  • Elicitation
  • Polygonum minus
  • Secondary metabolite
  • Suspension culture

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Agronomy and Crop Science

Cite this

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title = "Development of a Polygonum minus cell suspension culture system and analysis of secondary metabolites enhanced by elicitation",
abstract = "Polygonum minus has been reported to contain valuable metabolites and to date, there is no report on using cell culture technique for metabolite production in P. minus. Naphthalene acetic acid (NAA) concentrations in the range of 2-6 mg L-1 were used in a matrix of combinations with dichlorophenoxyacetic acid (2,4-D) concentrations in the range of 2-10 mg L-1 as plant growth regulators (PGRs) to induce callus cultures. Media that were supplemented with 2 mg L-1 2,4-D + 4 mg L-1 NAA, 2 mg L-1 2,4-D + 6 mg L-1 NAA and 6 mg L-1 2,4-D + 8 mg L-1 NAA were effective for callus induction (93. 3 {\%} of the explants produced callus). To establish cell culture, the best growth was obtained from medium that was supplemented with 1 mg L-1 2,4-D + 2 mg L-1 NAA. From a 1-g inoculum size, the fresh weight increases exponentially after 5-10 days of culture, and a 26. 71 g maximum fresh weight was obtained after 25 days of culture. The cell culture medium was then analyzed using gas chromatography-mass spectrometry (GC-MS). Jasmonic acid (100, 50, 25 and 5 μM), salicylic acid (100, 50, 25 and 5 μM), yeast extract (500, 250 and 100 mg L-1) and glass beads were used in this research as elicitors. The cell cultures were then incubated with the different elicitors for 1, 2, 3 and 4 days. Several compounds with high peak area percentages were detected, including 2-furancarboxaldehyde, 5-hydroxymethyl, furfural, and 2-cyclopenten-1-one, 2-hydroxy. These results show the diversity of metabolites released by P. minus cell into the culture medium under control conditions.",
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author = "Shukor, {Muhammad Faizan A} and Ismanizan Ismail and Zamri Zainal and {Mohd. Noor}, Normah",
year = "2013",
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volume = "35",
pages = "1675--1689",
journal = "Acta Physiologiae Plantarum",
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T1 - Development of a Polygonum minus cell suspension culture system and analysis of secondary metabolites enhanced by elicitation

AU - Shukor, Muhammad Faizan A

AU - Ismail, Ismanizan

AU - Zainal, Zamri

AU - Mohd. Noor, Normah

PY - 2013

Y1 - 2013

N2 - Polygonum minus has been reported to contain valuable metabolites and to date, there is no report on using cell culture technique for metabolite production in P. minus. Naphthalene acetic acid (NAA) concentrations in the range of 2-6 mg L-1 were used in a matrix of combinations with dichlorophenoxyacetic acid (2,4-D) concentrations in the range of 2-10 mg L-1 as plant growth regulators (PGRs) to induce callus cultures. Media that were supplemented with 2 mg L-1 2,4-D + 4 mg L-1 NAA, 2 mg L-1 2,4-D + 6 mg L-1 NAA and 6 mg L-1 2,4-D + 8 mg L-1 NAA were effective for callus induction (93. 3 % of the explants produced callus). To establish cell culture, the best growth was obtained from medium that was supplemented with 1 mg L-1 2,4-D + 2 mg L-1 NAA. From a 1-g inoculum size, the fresh weight increases exponentially after 5-10 days of culture, and a 26. 71 g maximum fresh weight was obtained after 25 days of culture. The cell culture medium was then analyzed using gas chromatography-mass spectrometry (GC-MS). Jasmonic acid (100, 50, 25 and 5 μM), salicylic acid (100, 50, 25 and 5 μM), yeast extract (500, 250 and 100 mg L-1) and glass beads were used in this research as elicitors. The cell cultures were then incubated with the different elicitors for 1, 2, 3 and 4 days. Several compounds with high peak area percentages were detected, including 2-furancarboxaldehyde, 5-hydroxymethyl, furfural, and 2-cyclopenten-1-one, 2-hydroxy. These results show the diversity of metabolites released by P. minus cell into the culture medium under control conditions.

AB - Polygonum minus has been reported to contain valuable metabolites and to date, there is no report on using cell culture technique for metabolite production in P. minus. Naphthalene acetic acid (NAA) concentrations in the range of 2-6 mg L-1 were used in a matrix of combinations with dichlorophenoxyacetic acid (2,4-D) concentrations in the range of 2-10 mg L-1 as plant growth regulators (PGRs) to induce callus cultures. Media that were supplemented with 2 mg L-1 2,4-D + 4 mg L-1 NAA, 2 mg L-1 2,4-D + 6 mg L-1 NAA and 6 mg L-1 2,4-D + 8 mg L-1 NAA were effective for callus induction (93. 3 % of the explants produced callus). To establish cell culture, the best growth was obtained from medium that was supplemented with 1 mg L-1 2,4-D + 2 mg L-1 NAA. From a 1-g inoculum size, the fresh weight increases exponentially after 5-10 days of culture, and a 26. 71 g maximum fresh weight was obtained after 25 days of culture. The cell culture medium was then analyzed using gas chromatography-mass spectrometry (GC-MS). Jasmonic acid (100, 50, 25 and 5 μM), salicylic acid (100, 50, 25 and 5 μM), yeast extract (500, 250 and 100 mg L-1) and glass beads were used in this research as elicitors. The cell cultures were then incubated with the different elicitors for 1, 2, 3 and 4 days. Several compounds with high peak area percentages were detected, including 2-furancarboxaldehyde, 5-hydroxymethyl, furfural, and 2-cyclopenten-1-one, 2-hydroxy. These results show the diversity of metabolites released by P. minus cell into the culture medium under control conditions.

KW - Elicitation

KW - Polygonum minus

KW - Secondary metabolite

KW - Suspension culture

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U2 - 10.1007/s11738-012-1210-9

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