Development of a modified method for sequencing high G: C regions in chicken anemia virus genome

S. M Z H Chowdhury, A. R. Omar, I. Aini, M. Hair-Bejo, A. A. Jamaluddin, Kiew Lian Wan, Y. Kono

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Two areas in the chicken anemia virus (CAV) genome have high G:C content with secondary structures. These two G:C rich areas could not be sequenced with Perkin Elmer's Big Dye Terminator Cycle Sequencing Kit. Several modifications were carried out to solve the problem. Finally, a package of modified method was developed to sequence the high G:C areas. The result showed that the Perkin Elmer's Big Dye Terminator Cycle Sequencing Kit with the normal procedures are not suitable for sequencing the high G:C regions of the CAV genome. The present developed method made the Perkin Elmer's Kit useful for the first time to sequence the G:C rich hairpin structures of the CAV genome. The system may be useful to sequence all other G:C rich DNA templates.

Original languageEnglish
Pages (from-to)229-232
Number of pages4
JournalJournal of Biochemistry, Molecular Biology and Biophysics
Volume6
Issue number3
DOIs
Publication statusPublished - Jun 2002

Fingerprint

Chicken anemia virus
Viruses
Genes
Genome
Coloring Agents
Base Composition
DNA

Keywords

  • Chicken anemia virus
  • Cloning
  • DNA templates
  • Sequencing G:C rich region

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics
  • Biophysics

Cite this

Development of a modified method for sequencing high G : C regions in chicken anemia virus genome. / Chowdhury, S. M Z H; Omar, A. R.; Aini, I.; Hair-Bejo, M.; Jamaluddin, A. A.; Wan, Kiew Lian; Kono, Y.

In: Journal of Biochemistry, Molecular Biology and Biophysics, Vol. 6, No. 3, 06.2002, p. 229-232.

Research output: Contribution to journalArticle

Chowdhury, S. M Z H ; Omar, A. R. ; Aini, I. ; Hair-Bejo, M. ; Jamaluddin, A. A. ; Wan, Kiew Lian ; Kono, Y. / Development of a modified method for sequencing high G : C regions in chicken anemia virus genome. In: Journal of Biochemistry, Molecular Biology and Biophysics. 2002 ; Vol. 6, No. 3. pp. 229-232.
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