Development of a fluorescence detection system for high-resolution melting analysis using silicon photomultiplier

K. S. Chong, N. A. Devi, S. M. Then, Gan Kok Beng

Research output: Contribution to journalArticle

Abstract

The introduction of fluorescent dye in the Polymerase Chain Reaction (PCR) assay has overcome the limitation of post-PCR analysis. Real-time PCR instruments have been developed to excite the fluorescent dye and detect the fluorescence light emits from the fluorescent dye during PCR and High-Resolution Melt (HRM) reactions. However, the cost and the size of existing benchtop instruments have limited its application for near patient testing. In this paper, a fluorescence detection system was designed and developed for high resolution melting analysis. The system used Light Emitting Diodes (LEDs) and Silicon Photomultipliers (SiPMs) as an excitation light source and fluorescent detector, respectively. The developed system can detect the fluorescence signal during the PCR and HRM processes. The development cost and the size of the system are lower and smaller compared to the real-time PCR instruments in the market. The developed system has overcome the limitation of existing real-time PCR instruments and improve the clinical facilities in developing country, especially for near patient testing.

Original languageEnglish
Pages (from-to)951-964
Number of pages14
JournalJournal of Engineering Science and Technology
Volume14
Issue number2
Publication statusPublished - 1 Apr 2019

Fingerprint

Polymerase chain reaction
Photomultipliers
Melting
Fluorescence
Silicon
Dyes
Testing
Developing countries
Light emitting diodes
Light sources
Costs
Assays
Detectors

Keywords

  • Fluorescence detection
  • High-resolution melting
  • Polymerase chain reaction
  • Real-time PCR
  • Silicon photomultiplier

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Development of a fluorescence detection system for high-resolution melting analysis using silicon photomultiplier. / Chong, K. S.; Devi, N. A.; Then, S. M.; Kok Beng, Gan.

In: Journal of Engineering Science and Technology, Vol. 14, No. 2, 01.04.2019, p. 951-964.

Research output: Contribution to journalArticle

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