Development and validation of HPLC analytical assay method for mefenamic acid tablet (ponstan)

Fouad Fadhil Al-Qaim, Md. Pauzi Abdullah, Mohamed Rozali Othman, Wan Mohd Afiq Wan Mohd Khalik

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Mefenamic acid is analgesic and anti-pyretic used to treat menstrual pain. A simple assay method by HPLC was developed and validated for mefenamic acid tablet (Ponstan). Analyses of mefenamic acid in a commercial tablet, Ponstan were performed using HPLC-Uv-Visbleat 275 nm on a reverse phase column Chromolith (RP-18e, 100 mm × 4.6 mm, 5 μm), a binary mobile phase; A: 0.1% formic acid in deionised water, B: 100% acetonitrile. The validation aspects were selectivity, linearity, precision, accuracy and quantification limit. Linearity, 5-250 mgL-1, provided determination coefficients (R2) of 0.9995, and proved precise since the RSD% was less than 5% for three replications analysis. The recoveries obtained ranged from 99% to 108%. In this study, the optimisation of mobile phase, flow rate, volume injection and wavelength were achieved through a statistical treatment. The retention time and drug content of mefenamic acid was 3.9 min and 97%, respectively. This method is precise, accurate and very simple to analysis mefenamic acid in tablets.

Original languageEnglish
Pages (from-to)62-72
Number of pages11
JournalInternational Journal of Chemical Sciences
Volume12
Issue number1
Publication statusPublished - 2014

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High-performance Liquid Chromatography
Linearity
Coefficient of Determination
Analgesics
Pain
Selectivity
Flow Rate
Quantification
Replication
Reverse
Injection
Drugs
Recovery
Wavelength
Binary
Water
Optimization
Mm

Keywords

  • HPLC assay method
  • Mefenamic acid
  • Pharmaceutical
  • Ponstan tablet

ASJC Scopus subject areas

  • Mathematics(all)

Cite this

Al-Qaim, F. F., Abdullah, M. P., Othman, M. R., & Khalik, W. M. A. W. M. (2014). Development and validation of HPLC analytical assay method for mefenamic acid tablet (ponstan). International Journal of Chemical Sciences, 12(1), 62-72.

Development and validation of HPLC analytical assay method for mefenamic acid tablet (ponstan). / Al-Qaim, Fouad Fadhil; Abdullah, Md. Pauzi; Othman, Mohamed Rozali; Khalik, Wan Mohd Afiq Wan Mohd.

In: International Journal of Chemical Sciences, Vol. 12, No. 1, 2014, p. 62-72.

Research output: Contribution to journalArticle

Al-Qaim, FF, Abdullah, MP, Othman, MR & Khalik, WMAWM 2014, 'Development and validation of HPLC analytical assay method for mefenamic acid tablet (ponstan)', International Journal of Chemical Sciences, vol. 12, no. 1, pp. 62-72.
Al-Qaim, Fouad Fadhil ; Abdullah, Md. Pauzi ; Othman, Mohamed Rozali ; Khalik, Wan Mohd Afiq Wan Mohd. / Development and validation of HPLC analytical assay method for mefenamic acid tablet (ponstan). In: International Journal of Chemical Sciences. 2014 ; Vol. 12, No. 1. pp. 62-72.
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