Development and in vitro assessment of alginate bilayer films containing the olive compound hydroxytyrosol as an alternative for topical chemotherapy

Shiow Fern Ng, Say Lee Tan

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Topical chemotherapy is the application of cancer drugs directly onto the skin, which has become a standard treatment for basal cell carcinoma. Due to the promising results in the treatment of skin cancer, topical chemotherapy has recently been applied to breast cancer patients because some breast cancer tissues are only superficial. Hydroxytyrosol, a phenolic compound from olives that is present in high amounts in Hidrox® olive extract, has been shown to have a protective effect on normal cells and selective antitumor activities on cancerous cells. The aims of the present study were to develop an alginate bilayer film containing Hidrox® and to investigate its potential use as a topical chemotherapeutic agent. Alginate films were characterized for swelling and for physical, thermal, rheological, and mechanical properties. Drug content uniformity and in vitro drug release tests were also investigated. The alginate bilayer films containing Hidrox®, HB2, showed controlled release of hydroxytyrosol at a flux of 0.094 ± 0.009 mg/cm2/h. The results of the cytotoxic assay showed that the HB2 films were dose-dependent and could significantly reduce the growth of breast cancer cells (MCF-7) at 150 μg/mL for a cell viability of 29.34 ± 4.64%. In conclusion, an alginate bilayer film containing Hidrox® can be a potential alternative for topical chemotherapeutic agent for skin and breast cancer treatment.

Original languageEnglish
Pages (from-to)798-806
Number of pages9
JournalInternational Journal of Pharmaceutics
Volume495
Issue number2
DOIs
Publication statusPublished - 30 Nov 2015

Fingerprint

Olea
Breast Neoplasms
Drug Therapy
Skin Neoplasms
Basal Cell Carcinoma
MCF-7 Cells
Pharmaceutical Preparations
Cell Survival
Therapeutics
Hot Temperature
Skin
3,4-dihydroxyphenylethanol
In Vitro Techniques
alginic acid
Growth
Neoplasms

Keywords

  • Alginate film
  • Breast cancer
  • Hidrox
  • Hydroxytyrosol
  • Topical chemotherapy

ASJC Scopus subject areas

  • Pharmaceutical Science

Cite this

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title = "Development and in vitro assessment of alginate bilayer films containing the olive compound hydroxytyrosol as an alternative for topical chemotherapy",
abstract = "Topical chemotherapy is the application of cancer drugs directly onto the skin, which has become a standard treatment for basal cell carcinoma. Due to the promising results in the treatment of skin cancer, topical chemotherapy has recently been applied to breast cancer patients because some breast cancer tissues are only superficial. Hydroxytyrosol, a phenolic compound from olives that is present in high amounts in Hidrox{\circledR} olive extract, has been shown to have a protective effect on normal cells and selective antitumor activities on cancerous cells. The aims of the present study were to develop an alginate bilayer film containing Hidrox{\circledR} and to investigate its potential use as a topical chemotherapeutic agent. Alginate films were characterized for swelling and for physical, thermal, rheological, and mechanical properties. Drug content uniformity and in vitro drug release tests were also investigated. The alginate bilayer films containing Hidrox{\circledR}, HB2, showed controlled release of hydroxytyrosol at a flux of 0.094 ± 0.009 mg/cm2/h. The results of the cytotoxic assay showed that the HB2 films were dose-dependent and could significantly reduce the growth of breast cancer cells (MCF-7) at 150 μg/mL for a cell viability of 29.34 ± 4.64{\%}. In conclusion, an alginate bilayer film containing Hidrox{\circledR} can be a potential alternative for topical chemotherapeutic agent for skin and breast cancer treatment.",
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