Determining the Pathogenic Potential of Non-sporulating Molds Isolated from Cutaneous Specimens

Nantha Kumar Jeyaprakasam, Mohd Fuat Abdul Razak, Noor Azimah Binti Ahmad, Jacinta Santhanam

Research output: Contribution to journalArticle

Abstract

Although non-sporulating molds (NSM) are frequently isolated from patients and have been recognized as agents of pulmonary disease, their clinical significance in cutaneous specimens is relatively unknown. Therefore, this study aimed to identify NSM and to determine the keratinolytic activity of isolates from cutaneous sites. NSM isolates from clinical specimens such as skin, nail, and body fluids were identified based on their ribosomal DNA sequences. Of 17 NSM isolates (7 Ascomycota, 10 Basidiomycota), eleven were identified to species level while five were identified to the genus level. These include Schizophyllum commune, a known human pathogen, Phoma multirostrata, a plant pathogen, and Perenniporia tephropora, a saprophyte. To determine fungal pathogenicity, keratinolytic activity, a major virulence factor, was evaluated ex vivo using human nail samples by measuring dye release from keratin azure, for NSM along with pathogens (Trichophyton mentagrophytes, Trichophyton rubrum, Microsporum canis and Fusarium spp.) and nonpathogenic (endophyte) fungi for comparison. This study showed that pathogenic fungi had the highest keratinolytic activity (7.13 ± 0.552 keratinase units) while the nonpathogenic endophytes had the lowest activity (2.37 ± 0.262 keratinase units). Keratinolytic activity of two Ascomycota NSM (Guignardia mangiferae and Hypoxylon sp.) and one Basidiomycota NSM (Fomitopsis cf. meliae) was equivalent to that of pathogenic fungi, while Xylaria feejeensis showed significantly higher activity (p 

Original languageEnglish
Pages (from-to)397-403
Number of pages7
JournalMycopathologia
Volume181
Issue number5-6
DOIs
Publication statusPublished - 1 Jun 2016

Fingerprint

molds (fungi)
Fungi
pathogenicity
Skin
nails (integument)
endophytes
Basidiomycota
Ascomycota
Endophytes
fungi
Trichophyton
Fomitopsis
Guignardia
Nails
Hypoxylon
Xylaria
Schizophyllum commune
Mangifera
saprophytes
Trichophyton rubrum

Keywords

  • Cutaneous
  • Dermatophytes
  • Keratinase enzyme
  • Non-sporulating molds
  • Pathogenicity

ASJC Scopus subject areas

  • Plant Science
  • Agronomy and Crop Science
  • Applied Microbiology and Biotechnology
  • Microbiology
  • veterinary (miscalleneous)

Cite this

Determining the Pathogenic Potential of Non-sporulating Molds Isolated from Cutaneous Specimens. / Jeyaprakasam, Nantha Kumar; Razak, Mohd Fuat Abdul; Ahmad, Noor Azimah Binti; Santhanam, Jacinta.

In: Mycopathologia, Vol. 181, No. 5-6, 01.06.2016, p. 397-403.

Research output: Contribution to journalArticle

Jeyaprakasam, Nantha Kumar ; Razak, Mohd Fuat Abdul ; Ahmad, Noor Azimah Binti ; Santhanam, Jacinta. / Determining the Pathogenic Potential of Non-sporulating Molds Isolated from Cutaneous Specimens. In: Mycopathologia. 2016 ; Vol. 181, No. 5-6. pp. 397-403.
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