Penggunaan kaedah tindak balas rantai polimerase dalam penentuan prognosis infeksi sistemik kandidiasis pada tikus

Translated title of the contribution: Determining prognosis of systemic candidiasis infection in rats with the polymerase chain reaction method

Jacinta Santhanam, Siti Azura Zainon, Chin Chook Fung, Faezah Shekh Abdullah

Research output: Contribution to journalArticle

Abstract

Candidiasis infections caused by the fungi Candida spp. are currently the most common systemic fungal infection in hospitalised patients. Diagnostic procedures involving culture or serological tests are either slow or lacking sensitivity and specificity. Thus polymerase chain reaction (PCR) assays which detect fungal DNA have been developed to provide a rapid, accurate diagnosis. In this study we evaluated a seminested PCR assay for the detection of systemic Candida albicans infection in laboratory animals. Adult Sprague-Dawley rats were infected with C. albicans by intravenous injection of yeast cells. Blood was collected from the animals every 3 to 4 days for extraction of DNA. Each week, 3 animals were sacrificed and their organs were cultured to confirm systemic C. albicans infection. The study period was 4 weeks (28 days). A seminested PCR was performed on DNA samples using universal fungal primers (ITS1 and ITS3) anda C. albicans specific primer (CALB1). The PCR product was detected by agarose gel electrophoresis. The PCR assay was able to detect C. albicans DNA in the blood samples of infected animals consistently from day 2 until day 25 post-infection. Organ culture of kidneys, liver and spleen revealed that the rats were systemically infected until day 21 post-infection and had recovered from infection (negative culture results) by day 28 post-infection. This study shows that the seminested PCR was an effective method to detect systemic C. albicans infection throughout the infection period. Therefore this assay is not only a diagnostic tool but has prognostic value as well for the monitoring of infection status.

Original languageUndefined/Unknown
Pages (from-to)321-327
Number of pages7
JournalSains Malaysiana
Volume39
Issue number2
Publication statusPublished - Apr 2010

Fingerprint

Candida albicans
Polymerase Chain Reaction
Infection
Mycoses
DNA
Fungal DNA
Agar Gel Electrophoresis
Candidiasis
Organ Culture Techniques
Laboratory Animals
Serologic Tests
Candida
Intravenous Injections
Sprague Dawley Rats
Systemic candidiasis
Spleen
Yeasts
Kidney
Sensitivity and Specificity
Liver

Keywords

  • Candida
  • Prognosis
  • Seminested
  • Systemic infection

ASJC Scopus subject areas

  • General

Cite this

Penggunaan kaedah tindak balas rantai polimerase dalam penentuan prognosis infeksi sistemik kandidiasis pada tikus. / Santhanam, Jacinta; Zainon, Siti Azura; Fung, Chin Chook; Abdullah, Faezah Shekh.

In: Sains Malaysiana, Vol. 39, No. 2, 04.2010, p. 321-327.

Research output: Contribution to journalArticle

Santhanam, Jacinta ; Zainon, Siti Azura ; Fung, Chin Chook ; Abdullah, Faezah Shekh. / Penggunaan kaedah tindak balas rantai polimerase dalam penentuan prognosis infeksi sistemik kandidiasis pada tikus. In: Sains Malaysiana. 2010 ; Vol. 39, No. 2. pp. 321-327.
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