Determination of the chromosome number and genome size of Garcinia mangostana L. via cytogenetics, flow cytometry and k-mer analyses

Mohd Razik Midin, Mohd Shukor Nordin, Maria Madon, Mohd Nazre Saleh, Hoe Han Goh, Normah Mohd. Noor

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Mangosteen (Garcinia mangostana L.) is one of the most popular tropical fruit of South-East Asia. It has considerable economic potential for local and export markets. This paper describes a research work to determine the number of chromosomes and genome size of G. mangostana through chromosome counting, flow cytometry and k-mer analyses. Chromosome count analysis revealed that the chromosome number of G. mangostana varied from 74 to 110. The high number observed could be due to the occurrence of mutation and aneuploidy in G. mangostana. Using flow cytometry with Glycine max cv. Polanka (2C = 2.5 pg) used as standard, G. mangostana genome size was found to be 2C = 6.00 ± 0.17 pg. Meanwhile, a genome survey of G. mangostana was performed using Illumina HiSeq 2000 DNA sequencing; k-mer analysis revealed that the genome size of G. mangostana was approximately 5.92 Gbp, or approximately 6.05 pg (1 pg DNA = 0.9780 × 109 bp). Based on the flow cytometry and genome survey, the study concludes that the genome size of G. mangostana is between 6.00 and 6.05 pg.

Original languageEnglish
Pages (from-to)1-10
Number of pages10
JournalCaryologia
DOIs
Publication statusAccepted/In press - 17 Dec 2017

Fingerprint

Garcinia mangostana
Genome Size
cytogenetics
Cytogenetics
chromosome number
flow cytometry
Flow Cytometry
Chromosomes
genome
Chromosomes, Human, 21-22 and Y
Genome
mangosteens
chromosomes
tropical and subtropical fruits
Far East
aneuploidy
Aneuploidy
DNA Sequence Analysis
Soybeans
South East Asia

ASJC Scopus subject areas

  • Genetics
  • Agricultural and Biological Sciences(all)

Cite this

Determination of the chromosome number and genome size of Garcinia mangostana L. via cytogenetics, flow cytometry and k-mer analyses. / Midin, Mohd Razik; Nordin, Mohd Shukor; Madon, Maria; Saleh, Mohd Nazre; Goh, Hoe Han; Mohd. Noor, Normah.

In: Caryologia, 17.12.2017, p. 1-10.

Research output: Contribution to journalArticle

Midin, Mohd Razik ; Nordin, Mohd Shukor ; Madon, Maria ; Saleh, Mohd Nazre ; Goh, Hoe Han ; Mohd. Noor, Normah. / Determination of the chromosome number and genome size of Garcinia mangostana L. via cytogenetics, flow cytometry and k-mer analyses. In: Caryologia. 2017 ; pp. 1-10.
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