Determination of Pediobius sp. (Hymenoptera: Eulophidae), a new species record of endoparasitoid associate with beet armyworm, Spodoptera exigua (Lepidoptera: Noctuidea) from Malaysia using DNA barcode

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2 Citations (Scopus)

Abstract

Spodoptera exigua (Lepidoptera: Noctuidea) is a polyphagous pest that attacks many important agricultural crops. Identifying an insect specimen is a crucial step in entomology. This study demonstrated a molecular method to identify the species of pest and its parasitoid in the case of a lack of a morphological identification key. To facilitate the identification of these pest-parasitoid species, a DNA barcoding approach was used for accurate and time-consuming identification based on nucleotide sequencing analysis of the mitochondrial Cytochrome Oxidase subunit I (COI) gene. DNA barcoding sequences successfully identified both pest and parasitoid species by comparing barcode sequences to the GenBank database. This study provided evidence of Pediobius sp. as a parasitoid of S. exigua in Malaysia.

Original languageEnglish
Pages (from-to)285-291
Number of pages7
JournalPertanika Journal of Tropical Agricultural Science
Volume37
Issue number2
Publication statusPublished - 2014

Fingerprint

Pediobius
Spodoptera exigua
Eulophidae
DNA barcoding
Malaysia
Hymenoptera
Lepidoptera
pests
new species
pest identification
taxonomic keys
barcoding
entomology
cytochrome-c oxidase
nucleotides
insects
crops
genes
methodology

Keywords

  • COI gene
  • DNA barcoding
  • Fern
  • Pediobius sp.
  • Spodoptera exigua

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

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title = "Determination of Pediobius sp. (Hymenoptera: Eulophidae), a new species record of endoparasitoid associate with beet armyworm, Spodoptera exigua (Lepidoptera: Noctuidea) from Malaysia using DNA barcode",
abstract = "Spodoptera exigua (Lepidoptera: Noctuidea) is a polyphagous pest that attacks many important agricultural crops. Identifying an insect specimen is a crucial step in entomology. This study demonstrated a molecular method to identify the species of pest and its parasitoid in the case of a lack of a morphological identification key. To facilitate the identification of these pest-parasitoid species, a DNA barcoding approach was used for accurate and time-consuming identification based on nucleotide sequencing analysis of the mitochondrial Cytochrome Oxidase subunit I (COI) gene. DNA barcoding sequences successfully identified both pest and parasitoid species by comparing barcode sequences to the GenBank database. This study provided evidence of Pediobius sp. as a parasitoid of S. exigua in Malaysia.",
keywords = "COI gene, DNA barcoding, Fern, Pediobius sp., Spodoptera exigua",
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AU - Ghazali, S. Z.

AU - Md. Zain, Badrul Munir

AU - Yaakop, Salmah

PY - 2014

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AB - Spodoptera exigua (Lepidoptera: Noctuidea) is a polyphagous pest that attacks many important agricultural crops. Identifying an insect specimen is a crucial step in entomology. This study demonstrated a molecular method to identify the species of pest and its parasitoid in the case of a lack of a morphological identification key. To facilitate the identification of these pest-parasitoid species, a DNA barcoding approach was used for accurate and time-consuming identification based on nucleotide sequencing analysis of the mitochondrial Cytochrome Oxidase subunit I (COI) gene. DNA barcoding sequences successfully identified both pest and parasitoid species by comparing barcode sequences to the GenBank database. This study provided evidence of Pediobius sp. as a parasitoid of S. exigua in Malaysia.

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