Determination of methylprednisolone acetate in biological fluids at gold nanoparticles modified ITO electrode

Rajendra N. Goyal, Munetaka Oyama, Ali Umar Akrajas, Anuradha Tyagi, Neeta Bachheti

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The electrochemical behavior of a corticosteroid methylprednisolone (MP), used for doping, has been studied at gold nanoparticles modified indium tin oxide (nanoAu/ITO) electrode. The nanoAu/ITO electrode exhibited an effective catalytic response towards its oxidation and lowered its oxidation potential by ∼127 mV when compared with bare ITO electrode. Oxidation of MP has been carried out in phosphate containing electrolyte in the pH range 2.13-10.00 and a well-defined oxidation peak was noticed. Linear concentration curves are obtained over the concentration range 0.01-1.0 μM with a detection limit of 2.68 × 10-7 M at nanoAu/ITO electrode. A diffusion coefficient of 2.36 × 10-6 cm2/s is calculated for MP using chronoamperometry. The proposed method is effectively applied to detect the concentration of MP in pharmaceutical formulations and human blood plasma and urine samples. A comparison of MP concentration determined in blood plasma and urine by the proposed method and GC/MS indicated that the results are essentially similar. It is believed that the method will be useful in determining this drug in case of doping.

Original languageEnglish
Pages (from-to)1147-1153
Number of pages7
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume44
Issue number5
DOIs
Publication statusPublished - 3 Sep 2007
Externally publishedYes

Fingerprint

Methylprednisolone
Gold
Nanoparticles
Electrodes
Fluids
Oxidation
Blood
Doping (additives)
Urine
Plasmas
Drug Compounding
Chronoamperometry
Pharmaceutical Preparations
Electrolytes
Limit of Detection
Adrenal Cortex Hormones
Phosphates
methylprednisolone acetate

Keywords

  • Blood plasma
  • Gold nanoparticles
  • Indium tin oxide
  • Methylprednisolone
  • Urine
  • Voltammetry

ASJC Scopus subject areas

  • Analytical Chemistry
  • Pharmaceutical Science

Cite this

Determination of methylprednisolone acetate in biological fluids at gold nanoparticles modified ITO electrode. / Goyal, Rajendra N.; Oyama, Munetaka; Akrajas, Ali Umar; Tyagi, Anuradha; Bachheti, Neeta.

In: Journal of Pharmaceutical and Biomedical Analysis, Vol. 44, No. 5, 03.09.2007, p. 1147-1153.

Research output: Contribution to journalArticle

Goyal, Rajendra N. ; Oyama, Munetaka ; Akrajas, Ali Umar ; Tyagi, Anuradha ; Bachheti, Neeta. / Determination of methylprednisolone acetate in biological fluids at gold nanoparticles modified ITO electrode. In: Journal of Pharmaceutical and Biomedical Analysis. 2007 ; Vol. 44, No. 5. pp. 1147-1153.
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