Determination of macrolide antibiotics in ChickenTissues by liquid chromatography-electrospray mass spectrometry

Jamilah Salikin, Aminah Abdullah

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

A methodusingliquid chromatography-electrospray mass spectrometry (LC-(ESI)MS) for the simultaneous determination of three macrolides (tylosin, spiramycin and tilmicosin) in poultry muscle has been developed. The drugs were extracted with EDTA McIlvaine buffer, filter through celite 545 and the extracts were cleaned up by SPE Oasis HLB cartridge. Separation was carried out in end-capped silica-based C18 column and mobile phases containing trifluoroacetic acid-acetonitrile with a binary gradient system at a flow rate 0.5 ml/min. Detection was performed by single mass spectrometry with electrospray ionization in the positive mode. Several parameters affecting the mass spectra were studied. Chicken samples from the market were analyzed to check the residue of macrolide antibiotics.

Original languageEnglish
Title of host publicationAIP Conference Proceedings
Pages702-709
Number of pages8
Volume1571
DOIs
Publication statusPublished - 2013
Event2013 UKM Faculty of Science and Technology Post-Graduate Colloquium - Selangor
Duration: 3 Jul 20134 Jul 2013

Other

Other2013 UKM Faculty of Science and Technology Post-Graduate Colloquium
CitySelangor
Period3/7/134/7/13

Fingerprint

antibiotics
liquid chromatography
mass spectroscopy
oases
cartridges
chickens
ethylenediaminetetraacetic acids
chromatography
muscles
mass spectra
acetonitrile
drugs
flow velocity
buffers
silicon dioxide
filters
ionization
gradients
acids

Keywords

  • Chicken muscle
  • Macrolide antibiotics
  • Mass spectrometry
  • Spiramycin
  • Tilmicosin
  • Tylosin

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

Determination of macrolide antibiotics in ChickenTissues by liquid chromatography-electrospray mass spectrometry. / Salikin, Jamilah; Abdullah, Aminah.

AIP Conference Proceedings. Vol. 1571 2013. p. 702-709.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Salikin, J & Abdullah, A 2013, Determination of macrolide antibiotics in ChickenTissues by liquid chromatography-electrospray mass spectrometry. in AIP Conference Proceedings. vol. 1571, pp. 702-709, 2013 UKM Faculty of Science and Technology Post-Graduate Colloquium, Selangor, 3/7/13. https://doi.org/10.1063/1.4858737
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