Determination of human epidermal growth factor receptor 2 (HER2) amplification using fluorescence in situ hybridization (FISH), chromogenic in situ hybridization (CISH) and automated silver in situ hybridization (SISH)

Nurhayati H. Munawer, Siti Aishah Md Ali, Reena Rahayu Md Zain, Munirah Mohd Ali, Rohaizak Muhammad, Noraidah Masir, Chandramaya S. Florence, K. Rafie Mohamed, Asmiati Arbi, Sharifah Noor Akmal, Srijit Das

Research output: Contribution to journalArticle

Abstract

Human epidermal growth factor receptor 2 (HER2) amplification is an important biomarker in breast carcinoma and its determination has been validated in the past using fluorescence in situ hybridization(FISH). However, HER2 FISH method requires specialized fluorescence microscope, high cost and impermanent signals causing it to be impractical for routine laboratories. The aim of the present study was to determine the expression of HER2 using FISH and another two recent new techniques, such as chromogenic in situ hybridization (CISH) and silver in situ hybridization (SISH). The whole sections of HER2 of 25 primary breast carcinoma cases with borderline positive (2+) immunohistochemistry (IHC) were further validated by using manual dual-color FISH, manual single color CISH and automated single color SISH. A total of 88, 84 and 92% of the cases showed HER2 amplification using FISH, CISH and SISH, respectively. A high level of concordance between FISH and CISH, FISH and SISH, CISH and SISH were observed in 91.6% (p = 0.032, k = 0.619), 96% (p = 0.01, k = 0.779) and 95.8% (p = 0.011, k = 0.778), respectively. SISH technique saved time, CISH equipment was less expensive and their high level of concordance shows that they may be alternatively used in routine laboratories.

Original languageEnglish
Pages (from-to)16429-16436
Number of pages8
JournalAfrican Journal of Biotechnology
Volume10
Issue number73
DOIs
Publication statusPublished - 21 Nov 2011

Fingerprint

fluorescence in situ hybridization
Fluorescence In Situ Hybridization
Silver
silver
in situ hybridization
In Situ Hybridization
breast neoplasms
erbB-2 receptor
human ERBB2 protein
color
Color
microscopes
immunohistochemistry
biomarkers
Breast Neoplasms
methodology
fluorescence

Keywords

  • Breast carcinoma
  • Chromogenic in situ hybridization (CISH)
  • Fluorescence in situ hybridization (FISH)
  • HER2
  • Immunohistochemistry
  • Silver in situ hybridization (SISH)

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Genetics
  • Molecular Biology
  • Agronomy and Crop Science

Cite this

Determination of human epidermal growth factor receptor 2 (HER2) amplification using fluorescence in situ hybridization (FISH), chromogenic in situ hybridization (CISH) and automated silver in situ hybridization (SISH). / Munawer, Nurhayati H.; Md Ali, Siti Aishah; Md Zain, Reena Rahayu; Mohd Ali, Munirah; Muhammad, Rohaizak; Masir, Noraidah; Florence, Chandramaya S.; Rafie Mohamed, K.; Arbi, Asmiati; Akmal, Sharifah Noor; Das, Srijit.

In: African Journal of Biotechnology, Vol. 10, No. 73, 21.11.2011, p. 16429-16436.

Research output: Contribution to journalArticle

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abstract = "Human epidermal growth factor receptor 2 (HER2) amplification is an important biomarker in breast carcinoma and its determination has been validated in the past using fluorescence in situ hybridization(FISH). However, HER2 FISH method requires specialized fluorescence microscope, high cost and impermanent signals causing it to be impractical for routine laboratories. The aim of the present study was to determine the expression of HER2 using FISH and another two recent new techniques, such as chromogenic in situ hybridization (CISH) and silver in situ hybridization (SISH). The whole sections of HER2 of 25 primary breast carcinoma cases with borderline positive (2+) immunohistochemistry (IHC) were further validated by using manual dual-color FISH, manual single color CISH and automated single color SISH. A total of 88, 84 and 92{\%} of the cases showed HER2 amplification using FISH, CISH and SISH, respectively. A high level of concordance between FISH and CISH, FISH and SISH, CISH and SISH were observed in 91.6{\%} (p = 0.032, k = 0.619), 96{\%} (p = 0.01, k = 0.779) and 95.8{\%} (p = 0.011, k = 0.778), respectively. SISH technique saved time, CISH equipment was less expensive and their high level of concordance shows that they may be alternatively used in routine laboratories.",
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AU - Md Zain, Reena Rahayu

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AU - Muhammad, Rohaizak

AU - Masir, Noraidah

AU - Florence, Chandramaya S.

AU - Rafie Mohamed, K.

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