Determination of host adaptation for wild highland population of Microgastrinae (Hymenoptera

Braconidae) using viral Histone H4

Ameyra Aman Zuki, Muhamad Azmi Mohammed, Badrul Munir Md. Zain, Salmah Yaakop

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Viral Histone H4 is a type of bracovirus gene that is highly conserved. It has a unique N-terminal that controls gene expression by chromatin remodeling. This region has been utilized to study the relationships between bracovirus and its parasitoid wasps. In this study, 10 individuals from seven significant species of Microgastrinae in highland ecosystems Cotesia, Apanteles sp. 1, Apanteles sp. 2, Apanteles sp. 3, Apanteles sp. 4, Dolichogenidae, and Choeras—were studied for relationships between the bracovirus and parasitoids using viral Histone H4, COI and 16S rRNA by using implementation of Bayesian inference (BI) analysis. We found that both trees were mutually incongruent, so we performed Phylogenetic Generalized Least Squares (PGLS) analysis to prove Histone H4 evolves independently, then reconfirmed the host adaptation of parasitoid towards host was driven by bracovirus and not vice versa. This study also demonstrates that the Histone H4 region is not suitable to study the relationships of the endosymbiont and its host. Nevertheless, these results provide new insight into the co-adaptation of bracovirus and its parasitoid towards the host, lepidopteran larvae, as well as providing the first ever samples of bracovirus from Malaysia.

Original languageEnglish
Pages (from-to)811-819
Number of pages9
JournalJournal of Asia-Pacific Entomology
Volume19
Issue number3
DOIs
Publication statusPublished - 1 Sep 2016

Fingerprint

Bracovirus
Braconidae
histones
Apanteles
highlands
Hymenoptera
Cotesia
endosymbionts
Bayesian theory
Malaysia
parasitoids
chromatin
least squares
Lepidoptera
ribosomal RNA
gene expression
ecosystems
larvae
phylogeny

Keywords

  • 16S
  • Bracovirus
  • COI
  • Histone H4
  • Host adaptation
  • Microgastrinae

ASJC Scopus subject areas

  • Insect Science

Cite this

Determination of host adaptation for wild highland population of Microgastrinae (Hymenoptera : Braconidae) using viral Histone H4. / Zuki, Ameyra Aman; Mohammed, Muhamad Azmi; Md. Zain, Badrul Munir; Yaakop, Salmah.

In: Journal of Asia-Pacific Entomology, Vol. 19, No. 3, 01.09.2016, p. 811-819.

Research output: Contribution to journalArticle

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