Determination of herbicide diuron levels in palm oil matrices using HPLC-UV

Mohd Izwarie Ramli, Zuriati Zakaria, Ismail Sahid, Tan Yew Ai, Halimah Muhamad

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The objective of this study was to develop a method for the determination of diuron (3-(3,4-dichlorophenyl)-1,1-dimethyl urea) residue in crude palm oil (CPO) and crude palm kernel oil (CPKO) matrices. The method involves the extraction of the herbicide from the oil matrix using low temperature precipitation and solid phase extraction techniques, detected by high performance liquid chromatography-ultra violet (HPLC-UV). The HPLC separation was carried out on an Ascentis™ RP-Amide column and elution with acetonitrile (solvent A) and water-methanol (2:1, v/v) (solvent B) as a suitable solvent system, at ratio of 4:6 (v/v). The optimum volume of acetonitrile for the extraction of diuron was 30 mL and 4 mL was obtained as the optimum volume of the solvent for elution analyte through the SPE cartridge. A linear correlation was obtained for the concentration of diuron from 0.05-1.0 μg mL -1 with a correlation coefficient of 0.99. The recovery of diuron from CPO was 83.2-101.4% with a relative standard deviation of 1.4-9.9% and 79.4-87.9% with relative standard deviation of 0.9-5.6% for CPKO. The method detection limit and limit of quantification obtained were 0.018 μg g -1 and 0.058 μg g -1, respectively. The method was used to determine diuron residues in palm oil from different refineries situated at different locations throughout Malaysia.

Original languageEnglish
Pages (from-to)1451-1459
Number of pages9
JournalSains Malaysiana
Volume41
Issue number11
Publication statusPublished - Nov 2012

Fingerprint

Diuron
High performance liquid chromatography
Herbicides
Amides
Methanol
Urea
Oils
palm oil
Recovery
Water

Keywords

  • Crude palm kernel oil
  • Crude palm oil
  • Diuron
  • Herbicide

ASJC Scopus subject areas

  • General

Cite this

Ramli, M. I., Zakaria, Z., Sahid, I., Ai, T. Y., & Muhamad, H. (2012). Determination of herbicide diuron levels in palm oil matrices using HPLC-UV. Sains Malaysiana, 41(11), 1451-1459.

Determination of herbicide diuron levels in palm oil matrices using HPLC-UV. / Ramli, Mohd Izwarie; Zakaria, Zuriati; Sahid, Ismail; Ai, Tan Yew; Muhamad, Halimah.

In: Sains Malaysiana, Vol. 41, No. 11, 11.2012, p. 1451-1459.

Research output: Contribution to journalArticle

Ramli, MI, Zakaria, Z, Sahid, I, Ai, TY & Muhamad, H 2012, 'Determination of herbicide diuron levels in palm oil matrices using HPLC-UV', Sains Malaysiana, vol. 41, no. 11, pp. 1451-1459.
Ramli MI, Zakaria Z, Sahid I, Ai TY, Muhamad H. Determination of herbicide diuron levels in palm oil matrices using HPLC-UV. Sains Malaysiana. 2012 Nov;41(11):1451-1459.
Ramli, Mohd Izwarie ; Zakaria, Zuriati ; Sahid, Ismail ; Ai, Tan Yew ; Muhamad, Halimah. / Determination of herbicide diuron levels in palm oil matrices using HPLC-UV. In: Sains Malaysiana. 2012 ; Vol. 41, No. 11. pp. 1451-1459.
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