Determination of haemolytic and Ematic genes profiles of bacillus cereus strains isolated from cooked rice samples by polymerase chain reaction (PCR) technique

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Abstract

The aim of this study has been an investigation of the presence of Bacillus cereus and detection of enterotoxigenic genes in cooked rice samples through utilizing a PCR technique. In this study the providence of B.cereus was carried out to cooked rice samples and the B.cereus isolates were investigated for enterotoxigenic gene. The cooked rice samples were purchased from several restaurants in the area of (Bangi, Kajang and UKM) Selangor, Malaysia. A total of 70 samples have been analyzed. B. cereus contamination has been formed between 1.2 × 104 to 1.6 × 106 cfu/g cooked of 110 colonies of tentative B. cereus have been tested onto mannitol egg yolk polymyxin agar and Chromogenic Bacillus cereus Agar, and 35 colonies have been detected as B. cereus using biochemical test and partial sequence of 16s r DNA sequences analysis. The B. cereus isolates that are BC1 to BC35 have been distinguished for hemolytic enterotoxin (HBL complex encoding gene (hblD), and ematic (ces) gene toxin. 12 isolates have been reported to be positive towards hblD, None of the B. cereus isolates have been found positive towards ematic(ces) gene. Therefore, the presence of B. cereus and their enterotoxigenic genes in cooked rice samples can be regarded as a potential risk for public health.

Original languageEnglish
Pages (from-to)193-198
Number of pages6
JournalInternational Journal of PharmTech Research
Volume8
Issue number7
Publication statusPublished - 2015

Fingerprint

Bacillus cereus
Polymerase Chain Reaction
Genes
Agar
Polymyxins
Restaurants
Egg Yolk
Oryza
Enterotoxins
Malaysia
Mannitol
DNA Sequence Analysis
Public Health

Keywords

  • B. cereus
  • Cooked rice
  • Ematic
  • Haemolytic
  • Isolated
  • PCR technique

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Pharmacology

Cite this

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title = "Determination of haemolytic and Ematic genes profiles of bacillus cereus strains isolated from cooked rice samples by polymerase chain reaction (PCR) technique",
abstract = "The aim of this study has been an investigation of the presence of Bacillus cereus and detection of enterotoxigenic genes in cooked rice samples through utilizing a PCR technique. In this study the providence of B.cereus was carried out to cooked rice samples and the B.cereus isolates were investigated for enterotoxigenic gene. The cooked rice samples were purchased from several restaurants in the area of (Bangi, Kajang and UKM) Selangor, Malaysia. A total of 70 samples have been analyzed. B. cereus contamination has been formed between 1.2 × 104 to 1.6 × 106 cfu/g cooked of 110 colonies of tentative B. cereus have been tested onto mannitol egg yolk polymyxin agar and Chromogenic Bacillus cereus Agar, and 35 colonies have been detected as B. cereus using biochemical test and partial sequence of 16s r DNA sequences analysis. The B. cereus isolates that are BC1 to BC35 have been distinguished for hemolytic enterotoxin (HBL complex encoding gene (hblD), and ematic (ces) gene toxin. 12 isolates have been reported to be positive towards hblD, None of the B. cereus isolates have been found positive towards ematic(ces) gene. Therefore, the presence of B. cereus and their enterotoxigenic genes in cooked rice samples can be regarded as a potential risk for public health.",
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T1 - Determination of haemolytic and Ematic genes profiles of bacillus cereus strains isolated from cooked rice samples by polymerase chain reaction (PCR) technique

AU - Jawad, Nisreen

AU - Abd. Mutalib, Sahilah

AU - Abdullah, Aminah

PY - 2015

Y1 - 2015

N2 - The aim of this study has been an investigation of the presence of Bacillus cereus and detection of enterotoxigenic genes in cooked rice samples through utilizing a PCR technique. In this study the providence of B.cereus was carried out to cooked rice samples and the B.cereus isolates were investigated for enterotoxigenic gene. The cooked rice samples were purchased from several restaurants in the area of (Bangi, Kajang and UKM) Selangor, Malaysia. A total of 70 samples have been analyzed. B. cereus contamination has been formed between 1.2 × 104 to 1.6 × 106 cfu/g cooked of 110 colonies of tentative B. cereus have been tested onto mannitol egg yolk polymyxin agar and Chromogenic Bacillus cereus Agar, and 35 colonies have been detected as B. cereus using biochemical test and partial sequence of 16s r DNA sequences analysis. The B. cereus isolates that are BC1 to BC35 have been distinguished for hemolytic enterotoxin (HBL complex encoding gene (hblD), and ematic (ces) gene toxin. 12 isolates have been reported to be positive towards hblD, None of the B. cereus isolates have been found positive towards ematic(ces) gene. Therefore, the presence of B. cereus and their enterotoxigenic genes in cooked rice samples can be regarded as a potential risk for public health.

AB - The aim of this study has been an investigation of the presence of Bacillus cereus and detection of enterotoxigenic genes in cooked rice samples through utilizing a PCR technique. In this study the providence of B.cereus was carried out to cooked rice samples and the B.cereus isolates were investigated for enterotoxigenic gene. The cooked rice samples were purchased from several restaurants in the area of (Bangi, Kajang and UKM) Selangor, Malaysia. A total of 70 samples have been analyzed. B. cereus contamination has been formed between 1.2 × 104 to 1.6 × 106 cfu/g cooked of 110 colonies of tentative B. cereus have been tested onto mannitol egg yolk polymyxin agar and Chromogenic Bacillus cereus Agar, and 35 colonies have been detected as B. cereus using biochemical test and partial sequence of 16s r DNA sequences analysis. The B. cereus isolates that are BC1 to BC35 have been distinguished for hemolytic enterotoxin (HBL complex encoding gene (hblD), and ematic (ces) gene toxin. 12 isolates have been reported to be positive towards hblD, None of the B. cereus isolates have been found positive towards ematic(ces) gene. Therefore, the presence of B. cereus and their enterotoxigenic genes in cooked rice samples can be regarded as a potential risk for public health.

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