Penentuan kandungan glukosa dalam Kenaf

Translated title of the contribution: Determination of glucose contents in Kenaf

Fatin Afifah Ahmad Kuthi, Nurulhuda Mohd Yunus, Goh Kae Horng, Khairiah Badri

Research output: Contribution to journalArticle

Abstract

Kenaf is one of the potential plant fibres used in research on the extraction and recovery of glucose. Kenaf fiber is comprised of a kenaf core (KC) and kenaf bast (KB). This study was performed to explore a hot water pre-treatment (HWP) using boiling water immersion for 2 hours on both KC and KB. Alkaline treatment using 6% (w/v) sodium hydroxide (NaOH) aqueous solution was subsequently performed for 3 hours at room temperature. The percentages of α-cellulose and hemicellulose in both KC and KB were found to increase after HWP. On the contrary, the percentages of α-cellulose and hemicellulose decreased upon NaOH treatment. The percentage of Klason lignin was reduced when KC and KB had undergone the HWP and NaOH treatments. A Fourier transform infrared spectroscopy analysis (FTIR) revealed the removal of lignin from both parts of kenaf fibre after the NaOH treatment. This was confirmed when the peaks at 1735 cm -1 and 1246 cm -1 disappeared after the treatment. Glucose as part of carbohydrates was determined in the fibre and filtrate using phenol sulfuric (PS) and dinitrosalicylic acid (DNS) analyses respectively. PS analysis indicated that the amount of glucose in KC-HWP (0.17 mg/g) was higher than in untreated KC (0.13 mg/g) and KC-NaOH (0.09 mg/g). The untreated KB showed the highest glucose content (0.36 mg/g), followed by KB-HWP (0.29 mg/g) and KB-NaOH (0.18 mg/g). Meanwhile, DNS analysis disclosed that the glucose concentrations in the KC filtrate for both treatments were 0.05 mg/mL. In contrast, the DNS analysis for KB showed a slightly lower glucose concentration in the KB-HWP filtrate (0.062 mg/mL) compared to the KB-NaOH (0.064 mg/mL). The glucose production was highly related to the composition of α-cellulose in the kenaf fibers.

Original languageMalay
Pages (from-to)1048-1056
Number of pages9
JournalMalaysian Journal of Analytical Sciences
Volume22
Issue number6
DOIs
Publication statusPublished - 1 Dec 2018

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Glucose
Kenaf fibers
Water
Cellulose
Lignin
Phenol
Acids
Sodium Hydroxide
Fibers
Boiling liquids
Carbohydrates
Recovery
Chemical analysis
Temperature
hemicellulose

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Penentuan kandungan glukosa dalam Kenaf. / Kuthi, Fatin Afifah Ahmad; Yunus, Nurulhuda Mohd; Horng, Goh Kae; Badri, Khairiah.

In: Malaysian Journal of Analytical Sciences, Vol. 22, No. 6, 01.12.2018, p. 1048-1056.

Research output: Contribution to journalArticle

Kuthi, Fatin Afifah Ahmad ; Yunus, Nurulhuda Mohd ; Horng, Goh Kae ; Badri, Khairiah. / Penentuan kandungan glukosa dalam Kenaf. In: Malaysian Journal of Analytical Sciences. 2018 ; Vol. 22, No. 6. pp. 1048-1056.
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abstract = "Kenaf is one of the potential plant fibres used in research on the extraction and recovery of glucose. Kenaf fiber is comprised of a kenaf core (KC) and kenaf bast (KB). This study was performed to explore a hot water pre-treatment (HWP) using boiling water immersion for 2 hours on both KC and KB. Alkaline treatment using 6{\%} (w/v) sodium hydroxide (NaOH) aqueous solution was subsequently performed for 3 hours at room temperature. The percentages of α-cellulose and hemicellulose in both KC and KB were found to increase after HWP. On the contrary, the percentages of α-cellulose and hemicellulose decreased upon NaOH treatment. The percentage of Klason lignin was reduced when KC and KB had undergone the HWP and NaOH treatments. A Fourier transform infrared spectroscopy analysis (FTIR) revealed the removal of lignin from both parts of kenaf fibre after the NaOH treatment. This was confirmed when the peaks at 1735 cm -1 and 1246 cm -1 disappeared after the treatment. Glucose as part of carbohydrates was determined in the fibre and filtrate using phenol sulfuric (PS) and dinitrosalicylic acid (DNS) analyses respectively. PS analysis indicated that the amount of glucose in KC-HWP (0.17 mg/g) was higher than in untreated KC (0.13 mg/g) and KC-NaOH (0.09 mg/g). The untreated KB showed the highest glucose content (0.36 mg/g), followed by KB-HWP (0.29 mg/g) and KB-NaOH (0.18 mg/g). Meanwhile, DNS analysis disclosed that the glucose concentrations in the KC filtrate for both treatments were 0.05 mg/mL. In contrast, the DNS analysis for KB showed a slightly lower glucose concentration in the KB-HWP filtrate (0.062 mg/mL) compared to the KB-NaOH (0.064 mg/mL). The glucose production was highly related to the composition of α-cellulose in the kenaf fibers.",
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AB - Kenaf is one of the potential plant fibres used in research on the extraction and recovery of glucose. Kenaf fiber is comprised of a kenaf core (KC) and kenaf bast (KB). This study was performed to explore a hot water pre-treatment (HWP) using boiling water immersion for 2 hours on both KC and KB. Alkaline treatment using 6% (w/v) sodium hydroxide (NaOH) aqueous solution was subsequently performed for 3 hours at room temperature. The percentages of α-cellulose and hemicellulose in both KC and KB were found to increase after HWP. On the contrary, the percentages of α-cellulose and hemicellulose decreased upon NaOH treatment. The percentage of Klason lignin was reduced when KC and KB had undergone the HWP and NaOH treatments. A Fourier transform infrared spectroscopy analysis (FTIR) revealed the removal of lignin from both parts of kenaf fibre after the NaOH treatment. This was confirmed when the peaks at 1735 cm -1 and 1246 cm -1 disappeared after the treatment. Glucose as part of carbohydrates was determined in the fibre and filtrate using phenol sulfuric (PS) and dinitrosalicylic acid (DNS) analyses respectively. PS analysis indicated that the amount of glucose in KC-HWP (0.17 mg/g) was higher than in untreated KC (0.13 mg/g) and KC-NaOH (0.09 mg/g). The untreated KB showed the highest glucose content (0.36 mg/g), followed by KB-HWP (0.29 mg/g) and KB-NaOH (0.18 mg/g). Meanwhile, DNS analysis disclosed that the glucose concentrations in the KC filtrate for both treatments were 0.05 mg/mL. In contrast, the DNS analysis for KB showed a slightly lower glucose concentration in the KB-HWP filtrate (0.062 mg/mL) compared to the KB-NaOH (0.064 mg/mL). The glucose production was highly related to the composition of α-cellulose in the kenaf fibers.

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