Determination of enterotoxin genes in bacillus cereus from raw milk using multiplex polymerase chain reaction technique

K. Nisreen Jawad, Wan Syaidatul Aqma Binti Wan Mohd Noor, Fathul Karim Sahrani, Yook Heng Lee, Asmat Ahmad

Research output: Contribution to journalArticle

Abstract

A Gram-positive bacterium, which can usually be found in cultivating plants and soil, causing it commonly universal is regarded as Bacillus cereus. It is said that most strains present in B. cereus is specifically called the primary cause of food poisoning illnesses which is characterised by either emetic or diarrheal syndrome, the most essential purpose of the current research was, therefore, to isolate and identify the enterotoxin genes in B. cereus through using a multiplex polymerase chain reaction (mPCR)technique. To achieve this aim, thus B.cereus was isolated from raw milk then cultured on mannitol egg yolk polymyxin agar and chromogenic B. cereus agar. The samples were then examined using biochemical investigation then established using partial sequencing of 16SrDNA. Among 40 samples, 20 of them were found to contain hblC and hblD, a hemolytic enterotoxin (HBL) complex encoding genes. The total mean of contamination samples in the B. cereus was proved to be higher (1.4×10 5 – 3.2×10 5 CFU/mL) than the acceptable detection level of B. cereus in dairy products ~10 3 CFU/mL. The isolates (10%) were shown to express hblC gene whereas the isolates (20%) were shown to express the hblD gene. The findings suggested that the detected enterotoxin genes of B. cereus which were found in raw milk may create a potential danger to the health of the public.

Original languageEnglish
Pages (from-to)645-649
Number of pages5
JournalInternational Journal of Research in Pharmaceutical Sciences
Volume10
Issue number1
DOIs
Publication statusPublished - 10 Jan 2019

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Bacillus cereus
Enterotoxins
Multiplex Polymerase Chain Reaction
Milk
Genes
Agar
Polymyxins
Emetics
Egg Yolk
Foodborne Diseases
Dairy Products
Gram-Positive Bacteria
Mannitol
Soil
Public Health
Research

Keywords

  • Bacillus cereus
  • Enterotoxin genes
  • Multiplex polymerase chain reaction
  • Raw milk

ASJC Scopus subject areas

  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Determination of enterotoxin genes in bacillus cereus from raw milk using multiplex polymerase chain reaction technique. / Nisreen Jawad, K.; Aqma Binti Wan Mohd Noor, Wan Syaidatul; Sahrani, Fathul Karim; Lee, Yook Heng; Ahmad, Asmat.

In: International Journal of Research in Pharmaceutical Sciences, Vol. 10, No. 1, 10.01.2019, p. 645-649.

Research output: Contribution to journalArticle

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