Penentuan kafien di permukaan air mengunakan pengekstrakan fasa pepejal dan kromatografi cecair prestasi tinggi

Translated title of the contribution: Determination of caffeine in surface water using solid phase extraction and high perfomance liquid chromatography

Fouad Fadhil Al-Qaim, Siti Hasmah Jusof, Md. Pauzi Abdullah, Zainab Haider Mussa, Nurfaizah Abu Tahrim, Wan Mohd Afiq Wan Mohd Khalik, Mohamed Rozali Othman

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A new analytical method development based on solid phase extraction (SPE) combined with high performance liquid chromatography (HPLC) was carried out. The optimum working conditions were obtained based on selection of 250 mL sample loading, 0.25 μL methanol as reconstitution solvent, 100% methanol as mobile phase and 270 nm as the optimum wavelength. Good linearity was obtained in the range of 0.015 – 400 mg/L and the regression coefficient, R2, was 0.995. Limit of detection and quantification were calculated at LOD = 0.06 μg/L and LOQ = 0.2 μg/L respectively. Repeatability and robustness has showed good performance with low relative standard deviation less than 3.29% and 3.50% respectively. Time-of-flight mass spectrometry (TOF/MS) instrument was used to confirm that caffeine is definitely present in surface water with level of concentration ranged from 31.7 to 50.1 μg/L. All results were analyzed statistically using one-way ANOVA, Tukey with interval confidence 95% and P-value 0.05.

Original languageMalay
Pages (from-to)95-104
Number of pages10
JournalMalaysian Journal of Analytical Sciences
Volume21
Issue number1
DOIs
Publication statusPublished - 1 Feb 2017

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Liquid chromatography
Caffeine
Surface waters
Methanol
High performance liquid chromatography
Analysis of variance (ANOVA)
Mass spectrometry
Wavelength

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Penentuan kafien di permukaan air mengunakan pengekstrakan fasa pepejal dan kromatografi cecair prestasi tinggi. / Al-Qaim, Fouad Fadhil; Jusof, Siti Hasmah; Abdullah, Md. Pauzi; Mussa, Zainab Haider; Tahrim, Nurfaizah Abu; Khalik, Wan Mohd Afiq Wan Mohd; Othman, Mohamed Rozali.

In: Malaysian Journal of Analytical Sciences, Vol. 21, No. 1, 01.02.2017, p. 95-104.

Research output: Contribution to journalArticle

Al-Qaim, Fouad Fadhil ; Jusof, Siti Hasmah ; Abdullah, Md. Pauzi ; Mussa, Zainab Haider ; Tahrim, Nurfaizah Abu ; Khalik, Wan Mohd Afiq Wan Mohd ; Othman, Mohamed Rozali. / Penentuan kafien di permukaan air mengunakan pengekstrakan fasa pepejal dan kromatografi cecair prestasi tinggi. In: Malaysian Journal of Analytical Sciences. 2017 ; Vol. 21, No. 1. pp. 95-104.
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