Detection of virulent Newcastle disease virus using a phage-capturing dot blot assay

Thong Chuan Lee, Khatijah Yusoff, Sheila Nathan, Wen Siang Tan

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Newcastle disease virus (NDV) strains can be classified as virulent or avirulent based upon the severity of the disease. Differentiation of the virus into virulent and avirulent is necessary for effective control of the disease. Biopanning experiments were performed using a disulfide constrained phage displayed heptapeptide library against three pathotypes of NDV strains: velogenic (highly virulent), mesogenic (moderately virulent) and lentogenic (avirulent). A phage clone bearing the peptide sequence SWGEYDM capable of distinguishing virulent from avirulent NDV strains was isolated. This phage clone was employed as a diagnostic reagent in a dot blot assay and it successfully detected only virulent NDV strains.

Original languageEnglish
Pages (from-to)224-229
Number of pages6
JournalJournal of Virological Methods
Volume136
Issue number1-2
DOIs
Publication statusPublished - Sep 2006

Fingerprint

Newcastle disease virus
Bacteriophages
Clone Cells
Disulfides
Libraries
Viruses
Peptides

Keywords

  • Dot blot
  • Filamentous phage
  • NDV
  • Pathotypes
  • Phage display

ASJC Scopus subject areas

  • Virology

Cite this

Detection of virulent Newcastle disease virus using a phage-capturing dot blot assay. / Lee, Thong Chuan; Yusoff, Khatijah; Nathan, Sheila; Tan, Wen Siang.

In: Journal of Virological Methods, Vol. 136, No. 1-2, 09.2006, p. 224-229.

Research output: Contribution to journalArticle

Lee, Thong Chuan ; Yusoff, Khatijah ; Nathan, Sheila ; Tan, Wen Siang. / Detection of virulent Newcastle disease virus using a phage-capturing dot blot assay. In: Journal of Virological Methods. 2006 ; Vol. 136, No. 1-2. pp. 224-229.
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