Detection of virulence genes and enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) analysis among raw vegetables isolates of Campylobacter jejuni

Sahilah Abd. Mutalib, T. S. Tuan Suraya, I. Noraida, A. Ahmad Azuhairi, L. C. Chai, R. Son

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A total of 20 (n=20) raw vegetable isolates of Campylobacter jejuni were examined for their virulence genes and genetic diversity by enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) analysis. All raw vegetable isolates of C. jejuni were encoded cadF and ceuE genes. None of the C. jejuni isolates was cdtA positive. Whereas, 12 (12/20) isolates were positive for cdtB and 6 (6/20) were positive for cdtC. All the 20 isolates of C. jejuni were subtyped and they produced 18 ERIC-PCR profiles namely E1 to E18. Dendrogram performed from cluster analysis showed that the 20 isolates were grouped into 2 major clusters, cluster I and II indicating the high level of local geographical genetic variation. The detection of virulence genes among C. jejuni strains isolated from raw vegetables raised concern on the threat of campylobacteriosis.

Original languageEnglish
Pages (from-to)681-690
Number of pages10
JournalInternational Food Research Journal
Volume17
Issue number3
Publication statusPublished - 2010

Fingerprint

Campylobacter jejuni
raw vegetables
Vegetables
Virulence
virulence
Polymerase Chain Reaction
Genes
genes
campylobacteriosis
genetic variation
Cluster Analysis
cluster analysis
polymerase chain reaction

Keywords

  • Campylobacter jejuni
  • ERIC-PCR
  • Raw vegetables
  • Virulence genes

ASJC Scopus subject areas

  • Food Science

Cite this

Detection of virulence genes and enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) analysis among raw vegetables isolates of Campylobacter jejuni. / Abd. Mutalib, Sahilah; Tuan Suraya, T. S.; Noraida, I.; Ahmad Azuhairi, A.; Chai, L. C.; Son, R.

In: International Food Research Journal, Vol. 17, No. 3, 2010, p. 681-690.

Research output: Contribution to journalArticle

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AU - Ahmad Azuhairi, A.

AU - Chai, L. C.

AU - Son, R.

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