Detection of Vibrio cholerae 01 from aquatic environment in Sarawak.

A. Norazah, M. T. Zainuldin, Mohamed Kamel Abdul Ghani, M. N. Kamaliah, A. M. Taha

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The detection of Vibrio cholerae 01 from the aquatic environment of Daro and Bintulu in Sarawak was carried out following an outbreak of cholera. Conventional culture methods and detection of ctx gene by polymerase chain reaction technique were carried out on 80 water samples. Only one sample was positive by culture methods while 8 were positive by PCR. DNA finger printing by pulsed-field gel electrophoresis showed that the clinical isolates in Daro and Bintulu were genetically identical while the environmental isolate was closely related. Recovery of Vibrio cholerae by culture method is poor and newer methods of detection should be developed.

Original languageEnglish
Pages (from-to)4-9
Number of pages6
JournalMedical Journal of Malaysia
Volume56
Issue number1
Publication statusPublished - Mar 2001
Externally publishedYes

Fingerprint

Vibrio cholerae
Malaysia
Polymerase Chain Reaction
Printing
Cholera
Pulsed Field Gel Electrophoresis
Fingers
Disease Outbreaks
Water
DNA
Genes

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Norazah, A., Zainuldin, M. T., Abdul Ghani, M. K., Kamaliah, M. N., & Taha, A. M. (2001). Detection of Vibrio cholerae 01 from aquatic environment in Sarawak. Medical Journal of Malaysia, 56(1), 4-9.

Detection of Vibrio cholerae 01 from aquatic environment in Sarawak. / Norazah, A.; Zainuldin, M. T.; Abdul Ghani, Mohamed Kamel; Kamaliah, M. N.; Taha, A. M.

In: Medical Journal of Malaysia, Vol. 56, No. 1, 03.2001, p. 4-9.

Research output: Contribution to journalArticle

Norazah, A, Zainuldin, MT, Abdul Ghani, MK, Kamaliah, MN & Taha, AM 2001, 'Detection of Vibrio cholerae 01 from aquatic environment in Sarawak.', Medical Journal of Malaysia, vol. 56, no. 1, pp. 4-9.
Norazah, A. ; Zainuldin, M. T. ; Abdul Ghani, Mohamed Kamel ; Kamaliah, M. N. ; Taha, A. M. / Detection of Vibrio cholerae 01 from aquatic environment in Sarawak. In: Medical Journal of Malaysia. 2001 ; Vol. 56, No. 1. pp. 4-9.
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