Detection of toluene degradation in bacteria isolated from oil contaminated soils

Ainon Hamzah, Arezoo Tavakoli, Amir Rabu

    Research output: Contribution to journalArticle

    13 Citations (Scopus)

    Abstract

    Toluene (C7H8) a hydrocarbon in crude oil, is a common contaminant in soil and groundwater. In this study, the ability to degrade toluene was investigated from twelve bacteria isolates which were isolated from soil contaminated with oil. Out of 12 bacterial isolates tested, most of Pseudomonas sp. showed the capability to grow in 1 mM of toluene compared with other isolates on the third day of incubation. Based on enzyme assays towards toluene monooxygenase, Pseudomonas aeruginosa UKMP-14T and Bacillus cereus UKMP-6G were shown to have the highest ability to degrade toluene. The toluene monoxygenase activity was analysed by using two calorimetric methods, Horseradish peroxidase (HRP) and indole-indigo. Both of the methods measured the production of catechol by the enzymatic reaction of toluene monooxygenase. In the HRP assay, the highest enzyme activity was 0.274 U/mL, exhibited by Pseudomonas aeruginosa UKMP-14T. However, for indole-indigo assay, Bacillus cereus UKMP-6G produced the highest enzyme activity of 0.291 U/ml. Results from both experiments showed that Pseudomonas aeruginosa UKMP-14T and Bacillus cereus UKMP-6G were able to degrade toluene.

    Original languageEnglish
    Pages (from-to)1231-1235
    Number of pages5
    JournalSains Malaysiana
    Volume40
    Issue number11
    Publication statusPublished - Nov 2011

    Fingerprint

    Toluene
    Oils
    Soil
    Bacteria
    Bacillus cereus
    Indigo Carmine
    Pseudomonas aeruginosa
    Horseradish Peroxidase
    Mixed Function Oxygenases
    Groundwater
    Petroleum
    Enzyme Assays
    Enzymes
    Hydrocarbons
    Pseudomonas

    Keywords

    • Degradation
    • Horseradish peroxidase indigo (HRP)
    • Indole
    • Toluene

    ASJC Scopus subject areas

    • General

    Cite this

    Hamzah, A., Tavakoli, A., & Rabu, A. (2011). Detection of toluene degradation in bacteria isolated from oil contaminated soils. Sains Malaysiana, 40(11), 1231-1235.

    Detection of toluene degradation in bacteria isolated from oil contaminated soils. / Hamzah, Ainon; Tavakoli, Arezoo; Rabu, Amir.

    In: Sains Malaysiana, Vol. 40, No. 11, 11.2011, p. 1231-1235.

    Research output: Contribution to journalArticle

    Hamzah, A, Tavakoli, A & Rabu, A 2011, 'Detection of toluene degradation in bacteria isolated from oil contaminated soils', Sains Malaysiana, vol. 40, no. 11, pp. 1231-1235.
    Hamzah, Ainon ; Tavakoli, Arezoo ; Rabu, Amir. / Detection of toluene degradation in bacteria isolated from oil contaminated soils. In: Sains Malaysiana. 2011 ; Vol. 40, No. 11. pp. 1231-1235.
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