Detection of TDH and TRH genes in Vibrio parahaemolyticus isolated from Corbicula moltkiana Prime in West Sumatera, Indonesia

Marlina, Son Radu, Cheah Yoke Kqueen, Suhaimi Napis, Zunita Zakaria, Sahilah Abd. Mutalib, Mitsuaki Nishibuchi

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The occurrence of Vibrio parahaemolytlcus in raw Corbicula moltkiana Prime from Lake Singkarak and Pasar Raya Padang market and in cooked samples in West Sumatera, Indonesia, was studied. Thirteen raw and seven cooked bivalve samples were positive using CHROMAgar™ Vibrio medium. All 47 V. parahaemolyticus isolates were positive for toxR gene but negative for trh. However, 36% (17/47) of V. parahaemolyticus strains were positive for tdh gene. Antibiotic profiling showed that 76% and 38% of isolates from raw and cooked bivalves respectively were resistant to ampiclllin. Using RAPD-PCR analysis, most of the strains were clustered according to their source of isolation but some of the strains from raw and cooked samples were clustered together. These results indicate that pathogenic V. parahaemolyticus isolates are present in Corbicula moltkiana Prime in West Sumatera, Indonesia, suggesting that V. parahaemolyticus may also be present in seafood In other regions of Indonesia.

Original languageEnglish
Pages (from-to)349-355
Number of pages7
JournalSoutheast Asian Journal of Tropical Medicine and Public Health
Volume38
Issue number2
Publication statusPublished - Mar 2007
Externally publishedYes

Fingerprint

Corbicula
Vibrio parahaemolyticus
Indonesia
Vibrio
Bivalvia
Genes
Random Amplified Polymorphic DNA Technique
Seafood
Lakes
Anti-Bacterial Agents

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Detection of TDH and TRH genes in Vibrio parahaemolyticus isolated from Corbicula moltkiana Prime in West Sumatera, Indonesia. / Marlina; Radu, Son; Kqueen, Cheah Yoke; Napis, Suhaimi; Zakaria, Zunita; Abd. Mutalib, Sahilah; Nishibuchi, Mitsuaki.

In: Southeast Asian Journal of Tropical Medicine and Public Health, Vol. 38, No. 2, 03.2007, p. 349-355.

Research output: Contribution to journalArticle

Marlina ; Radu, Son ; Kqueen, Cheah Yoke ; Napis, Suhaimi ; Zakaria, Zunita ; Abd. Mutalib, Sahilah ; Nishibuchi, Mitsuaki. / Detection of TDH and TRH genes in Vibrio parahaemolyticus isolated from Corbicula moltkiana Prime in West Sumatera, Indonesia. In: Southeast Asian Journal of Tropical Medicine and Public Health. 2007 ; Vol. 38, No. 2. pp. 349-355.
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