Detection of secreted antimicrobial peptides isolated from cell-free culture supernatant of Paenibacillus alvei AN5

Bassam Alkotaini, Nurina Anuar, Abdul Amir H. Kadhum, Asmahani Azira Abdu Sani

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

An antimicrobial substance produced by the Paenibacillus alvei strain AN5 was detected in fermentation broth. Subsequently, cell-free culture supernatant (CFCS) was obtained by medium centrifugation and filtration, and its antimicrobial activity was tested. This showed a broad inhibitory spectrum against both Gram-positive and -negative bacterial strains. The CFCS was then purified and subjected to SDS-PAGE and infrared spectroscopy, which indicated the proteinaceous nature of the antimicrobial compound. Some de novo sequencing using an automatic Q-TOF premier system determined the amino acid sequence of the purified antimicrobial peptide as Y-S-K-S-L-P-L-S-V-L-N-P (1,316 Da). The novel peptide was designated as peptide AN5-1. Its mode of action was bactericidal, inducing cell lysis in E. coli ATCC 29522 and S. aureus, and non-cell lysis in both S. marcescens and B. cereus ATCC 14579. Peptide AN5-1 displayed stability at a wide range of pH values (2-12) and remained active after exposure to high temperatures (100 C). It also maintained its antimicrobial activity after incubation with chemicals such as SDS, urea and EDTA.

Original languageEnglish
Pages (from-to)571-579
Number of pages9
JournalJournal of Industrial Microbiology and Biotechnology
Volume40
Issue number6
DOIs
Publication statusPublished - Jun 2013

Fingerprint

Paenibacillus
Peptides
Cell Culture Techniques
Centrifugation
Ethylenediaminetetraacetic acid
Urea
Fermentation
Escherichia coli
Amino acids
Polyacrylamide Gel Electrophoresis
Amino Acid Sequence
Infrared spectroscopy
Spectrum Analysis
Amino Acids
Temperature
gastrofenzin

Keywords

  • Action mode
  • Antimicrobial peptide AN5-1
  • LC/ESI-MS
  • Paenibacillus alvei AN5
  • Stability

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Detection of secreted antimicrobial peptides isolated from cell-free culture supernatant of Paenibacillus alvei AN5. / Alkotaini, Bassam; Anuar, Nurina; Kadhum, Abdul Amir H.; Sani, Asmahani Azira Abdu.

In: Journal of Industrial Microbiology and Biotechnology, Vol. 40, No. 6, 06.2013, p. 571-579.

Research output: Contribution to journalArticle

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