Detection of porcine dna in gelatin containing muffins using porcine gene chipTManalysis

Nursheila Mustafa Muin, Sahilah Abd. Mutalib, Aishah Elias, Norrakiah Abdullah Sani, Aminah Abdullah, Razalee Sedek, Izhar Ariff Mohd Kashim

Research output: Contribution to journalArticle

Abstract

This study was carried out to compare the sensitivity of two detection techniques using conventional polymerase chain reaction (PCR) and Porcine gene chip (OliproTM, Selangor, MY). Experimental samples consist of 15 muffins added with different percentage of porcine gelatin (1, 0.5, 0.1, 0.05, 0.01 and 0% w/w), and then cooked in two different ways (steamed and baked) were analyzed for the presence of porcine DNA in gelatin. Both Porcine gene chip and PCR analysis were targeting the gene of cytochrome b (cyt b). Porcine DNA detection in muffin samples using gene chip and PCR method were able to detect the presence of the porcine DNA at 73 and 53% of samples, respectively. Thus, this study demonstrated that the sensitivity level of porcine the DNA detection was high in Porcine Gene chip in comparisons with the PCR analysis.

Original languageEnglish
Pages (from-to)125-130
Number of pages6
JournalMalaysian Applied Biology
Volume48
Issue number2
Publication statusPublished - 1 May 2019

Fingerprint

muffins
Gelatin
gelatin
Swine
swine
Oligonucleotide Array Sequence Analysis
Genes
genes
polymerase chain reaction
Polymerase Chain Reaction
DNA
Cytochromes b
Gene Targeting
gene targeting
cytochrome b
sampling
DNA microarrays

Keywords

  • Gelatin-based products
  • PCR analysis
  • Porcine detection
  • Porcine Gene ChipTM

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Detection of porcine dna in gelatin containing muffins using porcine gene chipTManalysis. / Muin, Nursheila Mustafa; Abd. Mutalib, Sahilah; Elias, Aishah; Abdullah Sani, Norrakiah; Abdullah, Aminah; Sedek, Razalee; Kashim, Izhar Ariff Mohd.

In: Malaysian Applied Biology, Vol. 48, No. 2, 01.05.2019, p. 125-130.

Research output: Contribution to journalArticle

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