Detection of metals toxicity biosensor using immobilized cyanobacteria Anabaena flos-aquae

C. C. Tay, Salmijah Surif, Yook Heng Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

A whole-cell toxicity biosensor based on Anabaena flos-aquae for the determination of heavy metals was developed. Immobilized of Anabaena flos-aquae in a poly (2-hydroxyl ethyl methacrylate) (pHEMA) membrane interfaced with an oxygen electrode showed immediate response (within minutes) to toxicity of heavy metals (Cu & Pb). Response was measured in the form of inhibition of photosynthetic activity of the immobilized cyanobacteria through assessment of oxygen concentration changes. The 50% inhibition (IC50) of 0.4 mg/L and 0.6 mg/L of Cu were recorded for repetitive and single exposure respectively. The IC50 of Pb were slightly lower 0.3 mg/L and 0.5 mg/L in repetitive and single exposure respectively. The 50% inhibition of Cu and Pb in repetitive exposure and single exposure were significantly different statistically (p

Original languageEnglish
Title of host publication2003 Asian Conference on Sensors, AsiaSENSE 2003
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages197-201
Number of pages5
ISBN (Print)0780381017, 9780780381018
DOIs
Publication statusPublished - 2003
Event2003 Asian Conference on Sensors, AsiaSENSE 2003 - Kebangsann, Malaysia
Duration: 18 Jul 2003 → …

Other

Other2003 Asian Conference on Sensors, AsiaSENSE 2003
CountryMalaysia
CityKebangsann
Period18/7/03 → …

Fingerprint

Biosensors
Heavy metals
Toxicity
Oxygen
Metals
Membranes
Electrodes
Cyanobacteria

Keywords

  • Biochemistry
  • Biomembranes
  • Biosensors
  • Cells (biology)
  • Copper
  • Lead
  • Lighting
  • Microorganisms
  • Polymers
  • Stability

ASJC Scopus subject areas

  • Electrical and Electronic Engineering

Cite this

Tay, C. C., Surif, S., & Lee, Y. H. (2003). Detection of metals toxicity biosensor using immobilized cyanobacteria Anabaena flos-aquae. In 2003 Asian Conference on Sensors, AsiaSENSE 2003 (pp. 197-201). [1225018] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/ASENSE.2003.1225018

Detection of metals toxicity biosensor using immobilized cyanobacteria Anabaena flos-aquae. / Tay, C. C.; Surif, Salmijah; Lee, Yook Heng.

2003 Asian Conference on Sensors, AsiaSENSE 2003. Institute of Electrical and Electronics Engineers Inc., 2003. p. 197-201 1225018.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Tay, CC, Surif, S & Lee, YH 2003, Detection of metals toxicity biosensor using immobilized cyanobacteria Anabaena flos-aquae. in 2003 Asian Conference on Sensors, AsiaSENSE 2003., 1225018, Institute of Electrical and Electronics Engineers Inc., pp. 197-201, 2003 Asian Conference on Sensors, AsiaSENSE 2003, Kebangsann, Malaysia, 18/7/03. https://doi.org/10.1109/ASENSE.2003.1225018
Tay CC, Surif S, Lee YH. Detection of metals toxicity biosensor using immobilized cyanobacteria Anabaena flos-aquae. In 2003 Asian Conference on Sensors, AsiaSENSE 2003. Institute of Electrical and Electronics Engineers Inc. 2003. p. 197-201. 1225018 https://doi.org/10.1109/ASENSE.2003.1225018
Tay, C. C. ; Surif, Salmijah ; Lee, Yook Heng. / Detection of metals toxicity biosensor using immobilized cyanobacteria Anabaena flos-aquae. 2003 Asian Conference on Sensors, AsiaSENSE 2003. Institute of Electrical and Electronics Engineers Inc., 2003. pp. 197-201
@inproceedings{e4bbf8b50b9c46d78700bcc3ad6c93fe,
title = "Detection of metals toxicity biosensor using immobilized cyanobacteria Anabaena flos-aquae",
abstract = "A whole-cell toxicity biosensor based on Anabaena flos-aquae for the determination of heavy metals was developed. Immobilized of Anabaena flos-aquae in a poly (2-hydroxyl ethyl methacrylate) (pHEMA) membrane interfaced with an oxygen electrode showed immediate response (within minutes) to toxicity of heavy metals (Cu & Pb). Response was measured in the form of inhibition of photosynthetic activity of the immobilized cyanobacteria through assessment of oxygen concentration changes. The 50{\%} inhibition (IC50) of 0.4 mg/L and 0.6 mg/L of Cu were recorded for repetitive and single exposure respectively. The IC50 of Pb were slightly lower 0.3 mg/L and 0.5 mg/L in repetitive and single exposure respectively. The 50{\%} inhibition of Cu and Pb in repetitive exposure and single exposure were significantly different statistically (p",
keywords = "Biochemistry, Biomembranes, Biosensors, Cells (biology), Copper, Lead, Lighting, Microorganisms, Polymers, Stability",
author = "Tay, {C. C.} and Salmijah Surif and Lee, {Yook Heng}",
year = "2003",
doi = "10.1109/ASENSE.2003.1225018",
language = "English",
isbn = "0780381017",
pages = "197--201",
booktitle = "2003 Asian Conference on Sensors, AsiaSENSE 2003",
publisher = "Institute of Electrical and Electronics Engineers Inc.",

}

TY - GEN

T1 - Detection of metals toxicity biosensor using immobilized cyanobacteria Anabaena flos-aquae

AU - Tay, C. C.

AU - Surif, Salmijah

AU - Lee, Yook Heng

PY - 2003

Y1 - 2003

N2 - A whole-cell toxicity biosensor based on Anabaena flos-aquae for the determination of heavy metals was developed. Immobilized of Anabaena flos-aquae in a poly (2-hydroxyl ethyl methacrylate) (pHEMA) membrane interfaced with an oxygen electrode showed immediate response (within minutes) to toxicity of heavy metals (Cu & Pb). Response was measured in the form of inhibition of photosynthetic activity of the immobilized cyanobacteria through assessment of oxygen concentration changes. The 50% inhibition (IC50) of 0.4 mg/L and 0.6 mg/L of Cu were recorded for repetitive and single exposure respectively. The IC50 of Pb were slightly lower 0.3 mg/L and 0.5 mg/L in repetitive and single exposure respectively. The 50% inhibition of Cu and Pb in repetitive exposure and single exposure were significantly different statistically (p

AB - A whole-cell toxicity biosensor based on Anabaena flos-aquae for the determination of heavy metals was developed. Immobilized of Anabaena flos-aquae in a poly (2-hydroxyl ethyl methacrylate) (pHEMA) membrane interfaced with an oxygen electrode showed immediate response (within minutes) to toxicity of heavy metals (Cu & Pb). Response was measured in the form of inhibition of photosynthetic activity of the immobilized cyanobacteria through assessment of oxygen concentration changes. The 50% inhibition (IC50) of 0.4 mg/L and 0.6 mg/L of Cu were recorded for repetitive and single exposure respectively. The IC50 of Pb were slightly lower 0.3 mg/L and 0.5 mg/L in repetitive and single exposure respectively. The 50% inhibition of Cu and Pb in repetitive exposure and single exposure were significantly different statistically (p

KW - Biochemistry

KW - Biomembranes

KW - Biosensors

KW - Cells (biology)

KW - Copper

KW - Lead

KW - Lighting

KW - Microorganisms

KW - Polymers

KW - Stability

UR - http://www.scopus.com/inward/record.url?scp=84944724921&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84944724921&partnerID=8YFLogxK

U2 - 10.1109/ASENSE.2003.1225018

DO - 10.1109/ASENSE.2003.1225018

M3 - Conference contribution

AN - SCOPUS:84944724921

SN - 0780381017

SN - 9780780381018

SP - 197

EP - 201

BT - 2003 Asian Conference on Sensors, AsiaSENSE 2003

PB - Institute of Electrical and Electronics Engineers Inc.

ER -