Detection of immunogenic parasite and host-specific proteins in the sera of active and chronic individuals infected with Toxoplasma gondii

Chen Yeng, Emelia Osman, Zeehaida Mohamed, Rahmah Noordin

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Toxoplasma gondii infection in pregnant women may result in abortion and foetal abnormalities, and may be life-threatening in immunocompromised hosts. To identify the potential infection markers of this disease, 2-DE and Western blot methods were employed to study the parasite circulating antigens and host-specific proteins in the sera of T. gondii-infected individuals. The comparisons were made between serum protein profiles of infected (n=31) and normal (n=10) subjects. Antigenic proteins were identified by immunoblotting using pooled sera and monoclonal anti-human IgM-HRP. Selected protein spots were characterised using mass spectrometry. Prominent differences were observed when serum samples of T. gondii-infected individuals and normal controls were compared. A significant up-regulation of host-specific proteins, α2-HS glycoprotein and α1-B glycoprotein, was also observed in the silver-stained gels of both active and chronic infections. However, only α2-HS glycoprotein and α1-B glycoprotein in the active infection showed immunoreactivity in Western blots. In addition, three spots of T. gondii proteins were detected, namely (i) hypothetical protein chrXII: 3984434-3 TGME 49, (ii) dual specificity protein phosphatase, catalytic domain TGME 49 and (iii) NADPH-cytochrome p450 reductase TGME 49. Thus, 2-DE approach followed by Western blotting has enabled the identification of five potential infection markers for the diagnosis of toxoplasmosis: three are parasite-specific proteins and two are host-specific proteins.

Original languageEnglish
Pages (from-to)3843-3849
Number of pages7
JournalElectrophoresis
Volume31
Issue number23-24
DOIs
Publication statusPublished - Dec 2010
Externally publishedYes

Fingerprint

Toxoplasma
Blood Proteins
Parasites
Glycoproteins
Proteins
Western Blotting
Toxoplasmosis
Infection
Dual-Specificity Phosphatases
NADPH-Ferrihemoprotein Reductase
Phosphoprotein Phosphatases
Immunocompromised Host
Serum
Silver
Immunoblotting
Pregnant Women
Mass Spectrometry
Catalytic Domain
Up-Regulation
Mass spectrometry

Keywords

  • 2-DE
  • Host-specific protein
  • Immunoblot
  • Parasite protein
  • Toxoplasma gondii

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

Cite this

Detection of immunogenic parasite and host-specific proteins in the sera of active and chronic individuals infected with Toxoplasma gondii. / Yeng, Chen; Osman, Emelia; Mohamed, Zeehaida; Noordin, Rahmah.

In: Electrophoresis, Vol. 31, No. 23-24, 12.2010, p. 3843-3849.

Research output: Contribution to journalArticle

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