Detection of giardine gene in local isolates of Giardia duodenalis by polymerase chain reaction (PCR).

I. Latifah, K. Y. Teoh, Kiew Lian Wan, M. Rahmah, Y. Normaznah, A. Rohani

Research output: Contribution to journalArticle

Abstract

Giardia duodenalis is an intestinal parasite that causes diarrhoea and malabsorption in children. The parasite also infects AIDS patients with a weak immune system. A study was carried out on six local isolates of Giardia duodenalis (110, 7304, 6304, M007, 2002 and 6307) from faeces of Orang Asli patients admitted to the Gombak Hospital. WB, a reference pathogenic strain from human and G. muris from a wild mouse, were commercially obtained from the American Type Culture Collection (ATCC). All the isolates were cultured axenically in TYI-S-33 medium. Two sets of primers were used for the techniques: primers LP1 and RP1 and primers LP2 and RP2. The sets of primers amplified giardine gene of 171 bp and 218 bp in sizes respectively. The study showed that the two sets of primers could detect G. duodenalis to the genus and species level specifically.

Original languageEnglish
Pages (from-to)83-89
Number of pages7
JournalThe Malaysian journal of pathology
Volume27
Issue number2
Publication statusPublished - Dec 2005
Externally publishedYes

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Giardia lamblia
Polymerase Chain Reaction
Parasites
Genes
Feces
Diarrhea
Immune System
Acquired Immunodeficiency Syndrome

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Detection of giardine gene in local isolates of Giardia duodenalis by polymerase chain reaction (PCR). / Latifah, I.; Teoh, K. Y.; Wan, Kiew Lian; Rahmah, M.; Normaznah, Y.; Rohani, A.

In: The Malaysian journal of pathology, Vol. 27, No. 2, 12.2005, p. 83-89.

Research output: Contribution to journalArticle

Latifah, I. ; Teoh, K. Y. ; Wan, Kiew Lian ; Rahmah, M. ; Normaznah, Y. ; Rohani, A. / Detection of giardine gene in local isolates of Giardia duodenalis by polymerase chain reaction (PCR). In: The Malaysian journal of pathology. 2005 ; Vol. 27, No. 2. pp. 83-89.
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