Detection of CTX-M-type ESBLs Escherichia coli at universiti Kebangsaan Malaysia medical centre

Research output: Contribution to journalArticle

Abstract

Objective: To determine ESBLs and CTX-M-type ESBL-producing Escherichia coli at Universiti Kebangsaan Malaysia Medical Centre (UKMMC) by multiplex PCR. Materials and Methods: ESBL producing E. coli strains were confirmed by disk diffusion method. On the contrary, CTX-M-type ESBL-producing E.coli strains were confirmed by multiplex PCR. Results: Out of 554 collected E.coli isolates from UKMMC, 96 of these were detected as ESBL-producers. In 96 isolates, 76 viable strains were subjected to multiplex PCR for the detection of blaCTX genes. In which 70 (92.1%) were CTX-M-type ESBLs with the majority of CTX-M-1 group (77.1%), followed by CTX-M-9 group (21.4%) and one (1.4%) from CTX-M-2 group. Conclusions: CTX-Mtype ESBLs were the predominant ESBL types isolated at UKMMC. CTX-M-1 and-9 groups were found in majority of the clinical isolates. Although phenotypic characteristics based on disk diffusion test provided similar results, however, molecular detection of genes of organisms is important for further epidemiological investigations.

Original languageEnglish
Pages (from-to)257-261
Number of pages5
JournalBangladesh Journal of Medical Science
Volume15
Issue number2
Publication statusPublished - 2016

Fingerprint

Malaysia
Multiplex Polymerase Chain Reaction
Escherichia coli
Genes
beta-lactamase TEM-3

Keywords

  • CTX-M
  • ESBL
  • Escherichia coli
  • Multiplex PCR

ASJC Scopus subject areas

  • Medicine(all)

Cite this

@article{09b01912523043c5b3e8176a94872dad,
title = "Detection of CTX-M-type ESBLs Escherichia coli at universiti Kebangsaan Malaysia medical centre",
abstract = "Objective: To determine ESBLs and CTX-M-type ESBL-producing Escherichia coli at Universiti Kebangsaan Malaysia Medical Centre (UKMMC) by multiplex PCR. Materials and Methods: ESBL producing E. coli strains were confirmed by disk diffusion method. On the contrary, CTX-M-type ESBL-producing E.coli strains were confirmed by multiplex PCR. Results: Out of 554 collected E.coli isolates from UKMMC, 96 of these were detected as ESBL-producers. In 96 isolates, 76 viable strains were subjected to multiplex PCR for the detection of blaCTX genes. In which 70 (92.1{\%}) were CTX-M-type ESBLs with the majority of CTX-M-1 group (77.1{\%}), followed by CTX-M-9 group (21.4{\%}) and one (1.4{\%}) from CTX-M-2 group. Conclusions: CTX-Mtype ESBLs were the predominant ESBL types isolated at UKMMC. CTX-M-1 and-9 groups were found in majority of the clinical isolates. Although phenotypic characteristics based on disk diffusion test provided similar results, however, molecular detection of genes of organisms is important for further epidemiological investigations.",
keywords = "CTX-M, ESBL, Escherichia coli, Multiplex PCR",
author = "Othman, {Siti Norlia} and Salasawati Hussin and Ramliza Ramli and Rahman, {Md. Mostafizur}",
year = "2016",
language = "English",
volume = "15",
pages = "257--261",
journal = "Bangladesh Journal of Medical Science",
issn = "2223-4721",
publisher = "Ibn Sina Trust",
number = "2",

}

TY - JOUR

T1 - Detection of CTX-M-type ESBLs Escherichia coli at universiti Kebangsaan Malaysia medical centre

AU - Othman, Siti Norlia

AU - Hussin, Salasawati

AU - Ramli, Ramliza

AU - Rahman, Md. Mostafizur

PY - 2016

Y1 - 2016

N2 - Objective: To determine ESBLs and CTX-M-type ESBL-producing Escherichia coli at Universiti Kebangsaan Malaysia Medical Centre (UKMMC) by multiplex PCR. Materials and Methods: ESBL producing E. coli strains were confirmed by disk diffusion method. On the contrary, CTX-M-type ESBL-producing E.coli strains were confirmed by multiplex PCR. Results: Out of 554 collected E.coli isolates from UKMMC, 96 of these were detected as ESBL-producers. In 96 isolates, 76 viable strains were subjected to multiplex PCR for the detection of blaCTX genes. In which 70 (92.1%) were CTX-M-type ESBLs with the majority of CTX-M-1 group (77.1%), followed by CTX-M-9 group (21.4%) and one (1.4%) from CTX-M-2 group. Conclusions: CTX-Mtype ESBLs were the predominant ESBL types isolated at UKMMC. CTX-M-1 and-9 groups were found in majority of the clinical isolates. Although phenotypic characteristics based on disk diffusion test provided similar results, however, molecular detection of genes of organisms is important for further epidemiological investigations.

AB - Objective: To determine ESBLs and CTX-M-type ESBL-producing Escherichia coli at Universiti Kebangsaan Malaysia Medical Centre (UKMMC) by multiplex PCR. Materials and Methods: ESBL producing E. coli strains were confirmed by disk diffusion method. On the contrary, CTX-M-type ESBL-producing E.coli strains were confirmed by multiplex PCR. Results: Out of 554 collected E.coli isolates from UKMMC, 96 of these were detected as ESBL-producers. In 96 isolates, 76 viable strains were subjected to multiplex PCR for the detection of blaCTX genes. In which 70 (92.1%) were CTX-M-type ESBLs with the majority of CTX-M-1 group (77.1%), followed by CTX-M-9 group (21.4%) and one (1.4%) from CTX-M-2 group. Conclusions: CTX-Mtype ESBLs were the predominant ESBL types isolated at UKMMC. CTX-M-1 and-9 groups were found in majority of the clinical isolates. Although phenotypic characteristics based on disk diffusion test provided similar results, however, molecular detection of genes of organisms is important for further epidemiological investigations.

KW - CTX-M

KW - ESBL

KW - Escherichia coli

KW - Multiplex PCR

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