Pengesanan gen CollA2 odontoblas pada tisu pulpa gigi Oryctolagus cuniculus

Translated title of the contribution: Detection of CollA2 gene from Oryctolagus cuniculus pulp tissues

Shahrul Hisham Zainal Ariffin, Noreena Nordin, Foo Siah Li, Eni Juliana Ariffin, Sahidan Senafi, Rohaya Megat Abdul Wahab

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Type I collagen α - 2 gene is a member of collagen group that can be found in pulp tissues. Type I collagen constitutes about 56% of large intercellular spaces in the pulp. The objective of this study is to isolate the Col1A2 gene, directly from RNA isolation from pulp tissue Orycytolagus cuniculus. A housekeeping gene, i.e., GAPDH is used as apositive control gene. Specific primer sets for Col12 and GAPDH genes have been designed using Primer Premier Version 5 based on both gene sequences obtained from NCBI (National Center for Biotechnology Information) [www.ncbi.nlm.nih.gov]. RNA had been isolated directly from pulp tissues of Oryctolagus cuniculus. The PCR products for Col1A2 and GAPDH were successfully amplified with the size of 893 bp (Col1A2) and 229 bp (GAPDH). Sequencing results had shown that Col1A2 and GAPDH clone attained almost 99% similarity and 100% identical compared to the sequence in the NCBI database respectively. This study shows that Col1A2 gene has been successfully detected from pulp tissue of O. cuniculus.

Original languageUndefined/Unknown
Pages (from-to)261-269
Number of pages9
JournalSains Malaysiana
Volume38
Issue number2
Publication statusPublished - Apr 2009

Fingerprint

Oryctolagus cuniculus
pulp
National Center for Biotechnology Information
collagen
genes
RNA
intercellular spaces
tissues
clones
nucleotide sequences

Keywords

  • Dentine
  • Odontoblast
  • Oryctolagus cuniculus
  • Pulp tissues
  • RT-PCR

ASJC Scopus subject areas

  • General

Cite this

Pengesanan gen CollA2 odontoblas pada tisu pulpa gigi Oryctolagus cuniculus. / Zainal Ariffin, Shahrul Hisham; Nordin, Noreena; Li, Foo Siah; Ariffin, Eni Juliana; Senafi, Sahidan; Megat Abdul Wahab, Rohaya.

In: Sains Malaysiana, Vol. 38, No. 2, 04.2009, p. 261-269.

Research output: Contribution to journalArticle

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