Detection of Burkholderia pseudomallei toxin-mediated inhibition of protein synthesis using a Caenorhabditis elegans ugt-29 biosensor

Rui Rui Wong, Cin Kong, Song Hua Lee, Sheila Nathan

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Toxins are believed to play a crucial role in Burkholderia pseudomallei pathogenicity, however to date, only a few have been identified. The discovery of additional toxic molecules is limited by the lack of a sensitive indicator of B. pseudomallei toxicity. Previously, from a whole genome transcriptome analysis of B. pseudomallei-infected Caenorhabditis elegans, we noted significant overexpression of a number of worm genes encoding detoxification enzymes, indicating the host's attempt to clear bacterial toxic molecules. One of these genes, ugt-29, a family member of UDP-glucuronosyltransferases, was the most robustly induced phase II detoxification gene. In this study, we show that strong induction of ugt-29 is restricted to infections by the most virulent species among the pathogens tested. We also noted that ugt-29 is activated upon disruption of host protein synthesis. Hence, we propose that UGT-29 could be a promising biosensor to detect B. pseudomallei toxins that compromise host protein synthesis. The identification of bactobolin, a polyketide-peptide hybrid molecule, as a toxic molecule of B. pseudomallei further verifies the utilization of this surveillance system to search for bacterial toxins. Hence, a ugt-29 based reporter should be useful in screening for other molecules that inhibit host protein synthesis.

Original languageEnglish
Article number27475
JournalScientific Reports
Volume6
DOIs
Publication statusPublished - 7 Jun 2016

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Burkholderia pseudomallei
Caenorhabditis elegans
Biosensing Techniques
Poisons
Proteins
Phase II Metabolic Detoxication
Genes
Bacterial Toxins
Polyketides
Glucuronosyltransferase
Gene Expression Profiling
Virulence
Inhibition (Psychology)
Genome
Peptides
Enzymes
Infection

ASJC Scopus subject areas

  • General

Cite this

Detection of Burkholderia pseudomallei toxin-mediated inhibition of protein synthesis using a Caenorhabditis elegans ugt-29 biosensor. / Wong, Rui Rui; Kong, Cin; Lee, Song Hua; Nathan, Sheila.

In: Scientific Reports, Vol. 6, 27475, 07.06.2016.

Research output: Contribution to journalArticle

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