Detection of a Borrelia sp. From Ixodes granulatus Ticks Collected From Rodents in Malaysia

Jing Jing Khoo, Siti Nabilah Ishak, Fang Shiang Lim, Farah Shafawati Mohd Taib, Chee Sieng Khor, Shih Keng Loong, Sazaly AbuBakar

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The Borrelia genus consists of spirochete bacteria known to cause Lyme disease (LD) and relapsing fever in humans. Borrelia pathogens are commonly transmitted via arthropod vectors such as ticks, mites, or lice. Here, we report the molecular screening of LD group Borrelia sp. from ticks (Acari: Ixodidae) collected from rodents trapped in recreational forests and a semiurban residential area in the Selangor state in Malaysia. Of 156 adult ticks surveyed, 72 ticks were determined as positive for Borrelia sp. by polymerase chain reaction (PCR). All Borrelia PCR-positive ticks belonged to the Ixodes granulatus Supino species. Borrelia sp. was not detected in other tick species examined, including Dermacentor sp. and Amblyomma sp. ticks. Thirteen Borrelia PCR-positive tick samples were selected for further sequence analyses. Phylogenetic analyses of partial flaB gene sequences revealed that the Borrelia sp. were closely related to the LD group borreliae, Borrelia yangtzensis; a novel Borrelia genospecies reported in East Asian countries including Japan, Taiwan, and China. To our knowledge, this is the first report of Borrelia sp. related to Borrelia yangtzensis detected in Malaysia and Southeast Asia. The zoonotic potential of the Borrelia sp. reported here merits further investigation, as it may explain the previously reported serological evidence for borrelial infections in Malaysia.

Original languageEnglish
Pages (from-to)1642-1647
Number of pages6
JournalJournal of Medical Entomology
Volume55
Issue number6
DOIs
Publication statusPublished - 25 Oct 2018

Fingerprint

Borrelia
Ixodes
Malaysia
Ticks
ticks
Rodentia
rodents
Lyme disease
Lyme Disease
polymerase chain reaction
Polymerase Chain Reaction
Arthropod Vectors
Relapsing Fever
Dermacentor
Phthiraptera
Ixodidae
Amblyomma
Spirochaetales
Southeastern Asia
residential areas

ASJC Scopus subject areas

  • Parasitology
  • veterinary(all)
  • Insect Science
  • Infectious Diseases

Cite this

Detection of a Borrelia sp. From Ixodes granulatus Ticks Collected From Rodents in Malaysia. / Khoo, Jing Jing; Ishak, Siti Nabilah; Lim, Fang Shiang; Mohd Taib, Farah Shafawati; Khor, Chee Sieng; Loong, Shih Keng; AbuBakar, Sazaly.

In: Journal of Medical Entomology, Vol. 55, No. 6, 25.10.2018, p. 1642-1647.

Research output: Contribution to journalArticle

Khoo, Jing Jing ; Ishak, Siti Nabilah ; Lim, Fang Shiang ; Mohd Taib, Farah Shafawati ; Khor, Chee Sieng ; Loong, Shih Keng ; AbuBakar, Sazaly. / Detection of a Borrelia sp. From Ixodes granulatus Ticks Collected From Rodents in Malaysia. In: Journal of Medical Entomology. 2018 ; Vol. 55, No. 6. pp. 1642-1647.
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