Design of chitosan/PSf self-assembly membrane to mitigate fouling and enhance performance in trypsin separation

Sofiah Hamzah, Nora'aini Ali, Abdul Wahab Mohammad, Marinah Mohd Ariffin, Asmadi Ali

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Background: Membrane fouling in downstream processing has become a major obstacle in enzyme production. The use of a membrane with high surface hydrophilicity may be an acceptable way to overcome this problem. In this study, the effects of dip time on chitosan incorporation were investigated to produce a low fouling ultrafiltration membrane for trypsin separation. RESULT: Pristine ultrafiltration membrane with a polymer concentration of 15% was developed via phase inversion. Membrane surface modification was performed using chitosan solution with different dip times. Membranes with a 60-min dip time provided optimum trypsin transmission (about 91.8%). Such membranes have a high permeability coefficient (71 L m -2 h -1) and good porosity (about 89.6%). The hydrophilicity of this modified membrane was also improved by 50% compared with the native membrane, and its flux recovery was about 89.8%. The successful assembly of chitosan onto the membrane's surface was ascertained by ATR-FTIR and X-ray diffractometry (XRD). The morphology of this membrane was significantly different from that of native membrane. Conclusion: The experimental results show that membrane dip time exerts a significant influence on the self-assembly of chitosan particles onto the membrane's surface, and this process can be used to produce a potentially low-fouling UF membrane for trypsin separation.

Original languageEnglish
Pages (from-to)1157-1166
Number of pages10
JournalJournal of Chemical Technology and Biotechnology
Volume87
Issue number8
DOIs
Publication statusPublished - Aug 2012

Fingerprint

Chitosan
Fouling
fouling
Trypsin
Self assembly
membrane
Membranes
dip
Membrane fouling
Hydrophilicity
Ultrafiltration
ultrafiltration
Hydrophobic and Hydrophilic Interactions
Hydraulic conductivity
Porosity
X ray diffraction analysis
Fourier Transform Infrared Spectroscopy
Surface treatment
Polymers
Enzymes

Keywords

  • Chitosan
  • Fouling
  • Membranes
  • Trypsin
  • Ultrafiltration

ASJC Scopus subject areas

  • Inorganic Chemistry
  • Waste Management and Disposal
  • Pollution
  • Organic Chemistry
  • Renewable Energy, Sustainability and the Environment
  • Biotechnology
  • Chemical Engineering(all)
  • Fuel Technology

Cite this

Design of chitosan/PSf self-assembly membrane to mitigate fouling and enhance performance in trypsin separation. / Hamzah, Sofiah; Ali, Nora'aini; Mohammad, Abdul Wahab; Ariffin, Marinah Mohd; Ali, Asmadi.

In: Journal of Chemical Technology and Biotechnology, Vol. 87, No. 8, 08.2012, p. 1157-1166.

Research output: Contribution to journalArticle

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