Desiccation sensitivity and cryopreservation of excised embryonic axes of citrus suhuiensis cv. limau madu, citrumelo [Citrus paradisi Macf. x Poncirus trifoliata (L.) Raf.] and Fortunella polyandra tn Omar

M. Al Zoubi, Normah Mohd. Noor

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Excised embryonic axes from seeds of three taxa, namely, Citrus suhuiensis cv. limau madu, Citrumelo (Citrus paradisi x Poncirus trifoliate) and Fortunella polyandra, were desiccated in a laminar airflow, over silica gel, and ultra-rapidly. Desiccation sensitivity (WC 50) was estimated for each taxon using the quantal response model. High desiccation tolerance (WC 50 = 0.11 g H 2O g -1dw) was observed for limau madu embryonic axes desiccated in a laminar airflow and ultra-rapidly (WC 50 =0.10 g H 2O g -1dw). Desiccation tolerance was substantially lower (WC 50 = 0.19 g H 2O g -1dw) for silica gel dehydration. Similarly, high desiccation tolerance (WC 50 = 0.15 g H 2O g -1dw) was associated with F. polyandra embryonic axes when desiccated in a laminar airflow, while a lower desiccation tolerance (WC 50 = 0.17 g H 2O g -1dw) was observed with silica gel dehydration. Ultra-rapid desiccation led to the highest desiccation tolerance (WC 50 = 0.14 g H 2O g -1dw). The dehydration rate, however, had no influence on desiccation tolerance (WC 50 ∼0.14 g H 2O g -1 dw) for Citrumelo embryonic axes. After each desiccation period, embryonic axes were directly immersed in liquid nitrogen (LN) followed by rapid rewarming. Normal seedling recovery of 80 to 83% for excised embryonic axes of limau madu was observed for laminar airflow and ultra-rapid dehydration, but for silica gel dehydration, 57% recovery was obtained. Similarly, for Citrumelo, high recoveries of 100% and 97% were obtained from axes desiccated in a laminar airflow and using ultra-rapid dehydration, respectively, whereas a lower value was associated with silica gel dehydration (80%). For F. polyandra, 50% recovery was obtained both for laminar airflow and ultra-rapid dehydration, while much lower recovery (43%) was associated with silica gel dehydration. Regardless of the drying method employed, axis survival percentages following exposure to LN were commensurate with the desiccation sensitivity pattern.

Original languageEnglish
Pages (from-to)241-251
Number of pages11
JournalCryo-Letters
Volume33
Issue number3
Publication statusPublished - May 2012

Fingerprint

Poncirus
Fortunella
Rutaceae
Citrus paradisi
Desiccation
Poncirus trifoliata
Citrus
Cryopreservation
silica gel
air flow
cryopreservation
Dehydration
Silica Gel
liquids
nitrogen
Nitrogen
Rewarming
drying
Seedlings
seedlings

Keywords

  • Citrumelo (poncirus trifoliata x citrus paradisi)
  • Citrus suhuiensis cv. Limau madu
  • Cryopreservation
  • Dehydration rate
  • Desiccation sensitivity
  • Embryonic axis
  • Fortunella polyandra

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Anatomy

Cite this

@article{8afef101c1ed4dfdad71a235fab8052d,
title = "Desiccation sensitivity and cryopreservation of excised embryonic axes of citrus suhuiensis cv. limau madu, citrumelo [Citrus paradisi Macf. x Poncirus trifoliata (L.) Raf.] and Fortunella polyandra tn Omar",
abstract = "Excised embryonic axes from seeds of three taxa, namely, Citrus suhuiensis cv. limau madu, Citrumelo (Citrus paradisi x Poncirus trifoliate) and Fortunella polyandra, were desiccated in a laminar airflow, over silica gel, and ultra-rapidly. Desiccation sensitivity (WC 50) was estimated for each taxon using the quantal response model. High desiccation tolerance (WC 50 = 0.11 g H 2O g -1dw) was observed for limau madu embryonic axes desiccated in a laminar airflow and ultra-rapidly (WC 50 =0.10 g H 2O g -1dw). Desiccation tolerance was substantially lower (WC 50 = 0.19 g H 2O g -1dw) for silica gel dehydration. Similarly, high desiccation tolerance (WC 50 = 0.15 g H 2O g -1dw) was associated with F. polyandra embryonic axes when desiccated in a laminar airflow, while a lower desiccation tolerance (WC 50 = 0.17 g H 2O g -1dw) was observed with silica gel dehydration. Ultra-rapid desiccation led to the highest desiccation tolerance (WC 50 = 0.14 g H 2O g -1dw). The dehydration rate, however, had no influence on desiccation tolerance (WC 50 ∼0.14 g H 2O g -1 dw) for Citrumelo embryonic axes. After each desiccation period, embryonic axes were directly immersed in liquid nitrogen (LN) followed by rapid rewarming. Normal seedling recovery of 80 to 83{\%} for excised embryonic axes of limau madu was observed for laminar airflow and ultra-rapid dehydration, but for silica gel dehydration, 57{\%} recovery was obtained. Similarly, for Citrumelo, high recoveries of 100{\%} and 97{\%} were obtained from axes desiccated in a laminar airflow and using ultra-rapid dehydration, respectively, whereas a lower value was associated with silica gel dehydration (80{\%}). For F. polyandra, 50{\%} recovery was obtained both for laminar airflow and ultra-rapid dehydration, while much lower recovery (43{\%}) was associated with silica gel dehydration. Regardless of the drying method employed, axis survival percentages following exposure to LN were commensurate with the desiccation sensitivity pattern.",
keywords = "Citrumelo (poncirus trifoliata x citrus paradisi), Citrus suhuiensis cv. Limau madu, Cryopreservation, Dehydration rate, Desiccation sensitivity, Embryonic axis, Fortunella polyandra",
author = "{Al Zoubi}, M. and {Mohd. Noor}, Normah",
year = "2012",
month = "5",
language = "English",
volume = "33",
pages = "241--251",
journal = "Cryo-Letters",
issn = "0143-2044",
publisher = "Cryo-Letters",
number = "3",

}

TY - JOUR

T1 - Desiccation sensitivity and cryopreservation of excised embryonic axes of citrus suhuiensis cv. limau madu, citrumelo [Citrus paradisi Macf. x Poncirus trifoliata (L.) Raf.] and Fortunella polyandra tn Omar

AU - Al Zoubi, M.

AU - Mohd. Noor, Normah

PY - 2012/5

Y1 - 2012/5

N2 - Excised embryonic axes from seeds of three taxa, namely, Citrus suhuiensis cv. limau madu, Citrumelo (Citrus paradisi x Poncirus trifoliate) and Fortunella polyandra, were desiccated in a laminar airflow, over silica gel, and ultra-rapidly. Desiccation sensitivity (WC 50) was estimated for each taxon using the quantal response model. High desiccation tolerance (WC 50 = 0.11 g H 2O g -1dw) was observed for limau madu embryonic axes desiccated in a laminar airflow and ultra-rapidly (WC 50 =0.10 g H 2O g -1dw). Desiccation tolerance was substantially lower (WC 50 = 0.19 g H 2O g -1dw) for silica gel dehydration. Similarly, high desiccation tolerance (WC 50 = 0.15 g H 2O g -1dw) was associated with F. polyandra embryonic axes when desiccated in a laminar airflow, while a lower desiccation tolerance (WC 50 = 0.17 g H 2O g -1dw) was observed with silica gel dehydration. Ultra-rapid desiccation led to the highest desiccation tolerance (WC 50 = 0.14 g H 2O g -1dw). The dehydration rate, however, had no influence on desiccation tolerance (WC 50 ∼0.14 g H 2O g -1 dw) for Citrumelo embryonic axes. After each desiccation period, embryonic axes were directly immersed in liquid nitrogen (LN) followed by rapid rewarming. Normal seedling recovery of 80 to 83% for excised embryonic axes of limau madu was observed for laminar airflow and ultra-rapid dehydration, but for silica gel dehydration, 57% recovery was obtained. Similarly, for Citrumelo, high recoveries of 100% and 97% were obtained from axes desiccated in a laminar airflow and using ultra-rapid dehydration, respectively, whereas a lower value was associated with silica gel dehydration (80%). For F. polyandra, 50% recovery was obtained both for laminar airflow and ultra-rapid dehydration, while much lower recovery (43%) was associated with silica gel dehydration. Regardless of the drying method employed, axis survival percentages following exposure to LN were commensurate with the desiccation sensitivity pattern.

AB - Excised embryonic axes from seeds of three taxa, namely, Citrus suhuiensis cv. limau madu, Citrumelo (Citrus paradisi x Poncirus trifoliate) and Fortunella polyandra, were desiccated in a laminar airflow, over silica gel, and ultra-rapidly. Desiccation sensitivity (WC 50) was estimated for each taxon using the quantal response model. High desiccation tolerance (WC 50 = 0.11 g H 2O g -1dw) was observed for limau madu embryonic axes desiccated in a laminar airflow and ultra-rapidly (WC 50 =0.10 g H 2O g -1dw). Desiccation tolerance was substantially lower (WC 50 = 0.19 g H 2O g -1dw) for silica gel dehydration. Similarly, high desiccation tolerance (WC 50 = 0.15 g H 2O g -1dw) was associated with F. polyandra embryonic axes when desiccated in a laminar airflow, while a lower desiccation tolerance (WC 50 = 0.17 g H 2O g -1dw) was observed with silica gel dehydration. Ultra-rapid desiccation led to the highest desiccation tolerance (WC 50 = 0.14 g H 2O g -1dw). The dehydration rate, however, had no influence on desiccation tolerance (WC 50 ∼0.14 g H 2O g -1 dw) for Citrumelo embryonic axes. After each desiccation period, embryonic axes were directly immersed in liquid nitrogen (LN) followed by rapid rewarming. Normal seedling recovery of 80 to 83% for excised embryonic axes of limau madu was observed for laminar airflow and ultra-rapid dehydration, but for silica gel dehydration, 57% recovery was obtained. Similarly, for Citrumelo, high recoveries of 100% and 97% were obtained from axes desiccated in a laminar airflow and using ultra-rapid dehydration, respectively, whereas a lower value was associated with silica gel dehydration (80%). For F. polyandra, 50% recovery was obtained both for laminar airflow and ultra-rapid dehydration, while much lower recovery (43%) was associated with silica gel dehydration. Regardless of the drying method employed, axis survival percentages following exposure to LN were commensurate with the desiccation sensitivity pattern.

KW - Citrumelo (poncirus trifoliata x citrus paradisi)

KW - Citrus suhuiensis cv. Limau madu

KW - Cryopreservation

KW - Dehydration rate

KW - Desiccation sensitivity

KW - Embryonic axis

KW - Fortunella polyandra

UR - http://www.scopus.com/inward/record.url?scp=84864842819&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84864842819&partnerID=8YFLogxK

M3 - Article

VL - 33

SP - 241

EP - 251

JO - Cryo-Letters

JF - Cryo-Letters

SN - 0143-2044

IS - 3

ER -