Dental pulp stem cells therapy overcome photoreceptor cell death and protects the retina in a rat model of sodium iodate-induced retinal degeneration

Hiba Amer Alsaeedi, Avin Ee Hwan Koh, Chenshen Lam, Munirah Binti Abd Rashid, Mohd Hairul Nizam Harun, Muhamad Fakhri Bin Mohd Saleh, Seoh Wei Teh, C. D. Luu, Min Hwei Ng, Hazlita Mohd Isa, Sue Ngein Leow, Kong Yong Then, Mae-Lynn Catherine Bastion, Pooi Ling Mok, Bala Sundaram Muthuvenkatachalam, Antony V. Samrot, K. B. Swamy, Jaikumar Nandakumar, Suresh Subbiah Kumar

Research output: Contribution to journalArticle

Abstract

Blindness and vision loss contribute to irreversible retinal degeneration, and cellular therapy for retinal cell replacement has the potential to treat individuals who have lost light sensitive photoreceptors in the retina. Retinal cells are well characterized in function, and are a subject of interest in cellular replacement therapy of photoreceptors and the retinal pigment epithelium. However, retinal cell transplantation is limited by various factors, including the choice of potential stem cell source that can show variability in plasticity as well as host tissue integration. Dental pulp is one such source that contains an abundance of stem cells. In this study we used dental pulp-derived mesenchymal stem cells (DPSCs) to mitigate sodium iodate (NaIO3) insult in a rat model of retinal degeneration. Sprague-Dawley rats were first given an intravitreal injection of 3 × 105 DPSCs as well as a single systemic administration of NaIO3 (40 mg/kg). Electroretinography (ERG) was performed for the next two months and was followed-up by histological analysis. The ERG recordings showed protection of DPSC-treated retinas within 4 weeks, which was statistically significant (* P ≤ .05) compared to the control. Retinal thickness of the control was also found to be thinner (*** P ≤ .001). The DPSCs were found integrated in the photoreceptor layer through immunohistochemical staining. Our findings showed that DPSCs have the potential to moderate retinal degeneration. In conclusion, DPSCs are a potential source of stem cells in the field of eye stem cell therapy due to its protective effects against retinal degeneration.

Original languageEnglish
Article number111561
JournalJournal of Photochemistry and Photobiology B: Biology
Volume198
DOIs
Publication statusPublished - 1 Sep 2019

Fingerprint

iodates
Dental Pulp
photoreceptors
Photoreceptor Cells
Retinal Degeneration
degeneration
retina
stem cells
Cell- and Tissue-Based Therapy
death
rats
Retina
Mesenchymal Stromal Cells
therapy
Cell Death
Stem Cells
sodium
Electroretinography
electroretinography
Intravitreal Injections

Keywords

  • Dental pulp stem cells
  • Photoreceptors
  • Retinal degeneration
  • Retinal pigment epithelium
  • Sodium iodate

ASJC Scopus subject areas

  • Radiation
  • Radiological and Ultrasound Technology
  • Biophysics
  • Radiology Nuclear Medicine and imaging

Cite this

Dental pulp stem cells therapy overcome photoreceptor cell death and protects the retina in a rat model of sodium iodate-induced retinal degeneration. / Alsaeedi, Hiba Amer; Koh, Avin Ee Hwan; Lam, Chenshen; Rashid, Munirah Binti Abd; Harun, Mohd Hairul Nizam; Saleh, Muhamad Fakhri Bin Mohd; Teh, Seoh Wei; Luu, C. D.; Ng, Min Hwei; Mohd Isa, Hazlita; Leow, Sue Ngein; Then, Kong Yong; Bastion, Mae-Lynn Catherine; Mok, Pooi Ling; Muthuvenkatachalam, Bala Sundaram; Samrot, Antony V.; Swamy, K. B.; Nandakumar, Jaikumar; Kumar, Suresh Subbiah.

In: Journal of Photochemistry and Photobiology B: Biology, Vol. 198, 111561, 01.09.2019.

Research output: Contribution to journalArticle

Alsaeedi, HA, Koh, AEH, Lam, C, Rashid, MBA, Harun, MHN, Saleh, MFBM, Teh, SW, Luu, CD, Ng, MH, Mohd Isa, H, Leow, SN, Then, KY, Bastion, M-LC, Mok, PL, Muthuvenkatachalam, BS, Samrot, AV, Swamy, KB, Nandakumar, J & Kumar, SS 2019, 'Dental pulp stem cells therapy overcome photoreceptor cell death and protects the retina in a rat model of sodium iodate-induced retinal degeneration', Journal of Photochemistry and Photobiology B: Biology, vol. 198, 111561. https://doi.org/10.1016/j.jphotobiol.2019.111561
Alsaeedi, Hiba Amer ; Koh, Avin Ee Hwan ; Lam, Chenshen ; Rashid, Munirah Binti Abd ; Harun, Mohd Hairul Nizam ; Saleh, Muhamad Fakhri Bin Mohd ; Teh, Seoh Wei ; Luu, C. D. ; Ng, Min Hwei ; Mohd Isa, Hazlita ; Leow, Sue Ngein ; Then, Kong Yong ; Bastion, Mae-Lynn Catherine ; Mok, Pooi Ling ; Muthuvenkatachalam, Bala Sundaram ; Samrot, Antony V. ; Swamy, K. B. ; Nandakumar, Jaikumar ; Kumar, Suresh Subbiah. / Dental pulp stem cells therapy overcome photoreceptor cell death and protects the retina in a rat model of sodium iodate-induced retinal degeneration. In: Journal of Photochemistry and Photobiology B: Biology. 2019 ; Vol. 198.
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AU - Rashid, Munirah Binti Abd

AU - Harun, Mohd Hairul Nizam

AU - Saleh, Muhamad Fakhri Bin Mohd

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AU - Ng, Min Hwei

AU - Mohd Isa, Hazlita

AU - Leow, Sue Ngein

AU - Then, Kong Yong

AU - Bastion, Mae-Lynn Catherine

AU - Mok, Pooi Ling

AU - Muthuvenkatachalam, Bala Sundaram

AU - Samrot, Antony V.

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N2 - Blindness and vision loss contribute to irreversible retinal degeneration, and cellular therapy for retinal cell replacement has the potential to treat individuals who have lost light sensitive photoreceptors in the retina. Retinal cells are well characterized in function, and are a subject of interest in cellular replacement therapy of photoreceptors and the retinal pigment epithelium. However, retinal cell transplantation is limited by various factors, including the choice of potential stem cell source that can show variability in plasticity as well as host tissue integration. Dental pulp is one such source that contains an abundance of stem cells. In this study we used dental pulp-derived mesenchymal stem cells (DPSCs) to mitigate sodium iodate (NaIO3) insult in a rat model of retinal degeneration. Sprague-Dawley rats were first given an intravitreal injection of 3 × 105 DPSCs as well as a single systemic administration of NaIO3 (40 mg/kg). Electroretinography (ERG) was performed for the next two months and was followed-up by histological analysis. The ERG recordings showed protection of DPSC-treated retinas within 4 weeks, which was statistically significant (* P ≤ .05) compared to the control. Retinal thickness of the control was also found to be thinner (*** P ≤ .001). The DPSCs were found integrated in the photoreceptor layer through immunohistochemical staining. Our findings showed that DPSCs have the potential to moderate retinal degeneration. In conclusion, DPSCs are a potential source of stem cells in the field of eye stem cell therapy due to its protective effects against retinal degeneration.

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