Demethylbelamcandaquinone B isolated from Labisia Pumila enhanced proliferation and differentiation of osteoblast cells

Haryati Ahmad Hairi, Jamia Azdina Jamal, Nor Ashila Aladdin, Khairana Husin, Noor Suhaili Mohd Sofi, Norazlina Mohamed, Isa Naina Mohamed, Ahmad Nazrun Shuid

Research output: Contribution to journalArticle

Abstract

Labisia pumila (LP) or more commonly known as Kacip Fatimah in Malaysia has received much attention due to its estrogenic effects, including its role in the treatment of osteoporosis. This study was designed to explore the active compound of LP that may be responsible for its anti-osteoporotic effects. Crude aqueous extract of Labisia pumila var alata (LPva) was fractionated into hexane (Hex), dichloromethane (DCM) and methanol (Met) solvents and their proliferative effects on mouse osteoblastic cell line (MC3T3-E1) were evaluated with MTS bioassay. The DCM fraction significantly promoted cell proliferation in a dose-dependent manner. Thin layer chromatography (TLC) was performed on the DCM fraction of LPva to separate the constituents and the potential active compound was identified. Further isolation was achieved by column chromatography (CC) and Sephadex LH-20 column chromatography. The bioactivity of the isolated compound was confirmed by its ability to replicate MC3T3-E1 accelerated proliferation and differentiation. Mass spectrometry and nuclear magnetic resonance (NMR) identified the active compound as demethylbelamcandaquinone B. Further studies are required to determine the potential of this active compound of LPva in treating osteoporosis.

Original languageEnglish
Pages (from-to)12-20
Number of pages9
JournalJournal of Applied Pharmaceutical Science
Volume8
Issue number8
DOIs
Publication statusPublished - 1 Aug 2018

Fingerprint

Methylene Chloride
Osteoblasts
Cell Differentiation
Osteoporosis
Chromatography
Malaysia
Hexanes
Thin Layer Chromatography
Complex Mixtures
Biological Assay
Methanol
Mass Spectrometry
Estrogens
Magnetic Resonance Spectroscopy
Cell Proliferation
Cell Line
demethylbelamcandaquinone B

Keywords

  • Active compound
  • Labisia pumila
  • Osteoblasts
  • Osteoporosis

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Pharmacology (medical)

Cite this

Demethylbelamcandaquinone B isolated from Labisia Pumila enhanced proliferation and differentiation of osteoblast cells. / Hairi, Haryati Ahmad; Jamal, Jamia Azdina; Aladdin, Nor Ashila; Husin, Khairana; Sofi, Noor Suhaili Mohd; Mohamed, Norazlina; Naina Mohamed, Isa; Shuid, Ahmad Nazrun.

In: Journal of Applied Pharmaceutical Science, Vol. 8, No. 8, 01.08.2018, p. 12-20.

Research output: Contribution to journalArticle

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