Deleterious effects of nicotine on the ultrastructure of oocytes

Role of γ-tocotrienol

Mohd Hamim Rajikin, Elda Surhaida Latif, Megat Radzi Mar, A. Gapor Mat Top, Norfilza Mohd Mokhtar

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Background: Previous studies have shown that nicotine enhances oxidative DNA damage and leads to increased lipid peroxidation, which affects embryo development. The present study investigated the effect of daily supplementation of γ-tocotrienol on oocytes of nicotine-treated mice. Material/Methods: Immature female mice (18-25 g) were divided into three groups. For 30 days, group A (control group) received saline (0.2 ml/day s.c.), group B nicotine (5 mg/kg/day s.c. in saline), and group C nicotine with γ-tocotrienol (60 mg/kg/day p.o.). The animals were superovulated following these schedules. Results: Scanning electron microscopy (SEM) showed that the nicotine-treated oocytes appeared nonspherical with rough surface and the zona pellucida (zp) was torn and became irregular. Supplementation with γ-tocotrienol in the nicotine-treated mice retained the spherical shape of the oocytes with intact zp; however, the surfaces of the oocytes remained irregular and rough. Transmission electron microscopy (TEM) following chronic nicotine treatment showed loosening of the boundary and tearing of the zp. The perivitelline space was also widened. The cytoplasm of the oocytes retained abundant rough endoplasmic reticulum (rER) with numerous vesicles. Mitochondria were highly dense, with no cristae. The administration of γ-tocotrienol partially reduced the detrimental effects of nicotine by retaining the smooth boundary of the zp with the tight perivitelline space. There was less rER with no visible vesicle and a lower amount of dense mitochondrial matrix. Conclusions: This study documented that chronic nicotine treatment adversely affects the ultrastructure of oocytes, while γ-tocotrienol treatment at least minimizes the nicotine-induced damage to oocytes.

Original languageEnglish
JournalMedical Science Monitor
Volume15
Issue number12
Publication statusPublished - Dec 2009

Fingerprint

Tocotrienols
Nicotine
Oocytes
Zona Pellucida
Rough Endoplasmic Reticulum
Transmission Electron Microscopy
Electron Scanning Microscopy
Lipid Peroxidation
DNA Damage
Embryonic Development
Appointments and Schedules
Mitochondria
Cytoplasm

Keywords

  • γ-tocotrienol
  • Nicotine
  • Oocytes
  • Scanning electron microscope
  • Transmission electron microscopy
  • Ultrastructure

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Deleterious effects of nicotine on the ultrastructure of oocytes : Role of γ-tocotrienol. / Rajikin, Mohd Hamim; Latif, Elda Surhaida; Mar, Megat Radzi; Top, A. Gapor Mat; Mohd Mokhtar, Norfilza.

In: Medical Science Monitor, Vol. 15, No. 12, 12.2009.

Research output: Contribution to journalArticle

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abstract = "Background: Previous studies have shown that nicotine enhances oxidative DNA damage and leads to increased lipid peroxidation, which affects embryo development. The present study investigated the effect of daily supplementation of γ-tocotrienol on oocytes of nicotine-treated mice. Material/Methods: Immature female mice (18-25 g) were divided into three groups. For 30 days, group A (control group) received saline (0.2 ml/day s.c.), group B nicotine (5 mg/kg/day s.c. in saline), and group C nicotine with γ-tocotrienol (60 mg/kg/day p.o.). The animals were superovulated following these schedules. Results: Scanning electron microscopy (SEM) showed that the nicotine-treated oocytes appeared nonspherical with rough surface and the zona pellucida (zp) was torn and became irregular. Supplementation with γ-tocotrienol in the nicotine-treated mice retained the spherical shape of the oocytes with intact zp; however, the surfaces of the oocytes remained irregular and rough. Transmission electron microscopy (TEM) following chronic nicotine treatment showed loosening of the boundary and tearing of the zp. The perivitelline space was also widened. The cytoplasm of the oocytes retained abundant rough endoplasmic reticulum (rER) with numerous vesicles. Mitochondria were highly dense, with no cristae. The administration of γ-tocotrienol partially reduced the detrimental effects of nicotine by retaining the smooth boundary of the zp with the tight perivitelline space. There was less rER with no visible vesicle and a lower amount of dense mitochondrial matrix. Conclusions: This study documented that chronic nicotine treatment adversely affects the ultrastructure of oocytes, while γ-tocotrienol treatment at least minimizes the nicotine-induced damage to oocytes.",
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