Datasets from an interaction proteomics screen for substrates of the SCFβTrCP ubiquitin ligase

Roberto Magliozzi, Mao Peng, Shabaz Mohammed, Daniele Guardavaccaro, Albert J R Heck, Low Teck Yew

Research output: Contribution to journalArticle

Abstract

An affinity purification-mass spectrometry (AP-MS) method was employed to identify novel substrates of the SCFβTrCP ubiquitin ligase. A FLAG-HA tagged version of the F-box protein βTrCP2, the substrate recognition subunit of SCFβTrCP, was used as bait. βTrCP2 wild type and the two mutants βTrCP2-R447A and βTrCP2-δF were expressed and purified from HEK293T cells to be able to discriminate between potential substrates of SCFβTrCP and unspecific binders. Affinity-purified samples were analyzed by mass spectrometry-based proteomics, applying ultra-high performance liquid chromatography (UHPLC) coupled to high-resolution tandem mass spectrometry. The raw mass spectrometry data have been deposited to the PRIDE partner repository with the identifiers PXD001088 and PXD001224. The present dataset is associated with a research resource published in T.Y. Low, M. Peng, R. Magliozzi, S. Mohammed, D. Guardavaccaro, A.J.R. Heck, A systems-wide screen identifies substrates of the SCFβTrCP ubiquitin ligase. Sci. Signal. 7 (2014) rs8-rs8, 10.1126/scisignal.2005882.

Original languageEnglish
Pages (from-to)229-234
Number of pages6
JournalData in Brief
Volume4
DOIs
Publication statusPublished - 1 Sep 2015
Externally publishedYes

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Keywords

  • Affinity purification-mass spectrometry (AP-MS)
  • F-box protein
  • Proteomics
  • SCF ubiquitin ligase
  • βTrCP

ASJC Scopus subject areas

  • Education
  • General

Cite this

Datasets from an interaction proteomics screen for substrates of the SCFβTrCP ubiquitin ligase. / Magliozzi, Roberto; Peng, Mao; Mohammed, Shabaz; Guardavaccaro, Daniele; Heck, Albert J R; Teck Yew, Low.

In: Data in Brief, Vol. 4, 01.09.2015, p. 229-234.

Research output: Contribution to journalArticle

Magliozzi, Roberto ; Peng, Mao ; Mohammed, Shabaz ; Guardavaccaro, Daniele ; Heck, Albert J R ; Teck Yew, Low. / Datasets from an interaction proteomics screen for substrates of the SCFβTrCP ubiquitin ligase. In: Data in Brief. 2015 ; Vol. 4. pp. 229-234.
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