Cytotoxicity of nickel zinc ferrite nanoparticles on cancer cells of epithelial origin

Mothanna Sadiq Al-Qubaisi, Abdullah Rasedee, Moayad Husein Flaifel, Sahrim Ahmad, Samer Hussein-Al-Ali, Mohd Zobir Hussein, Eltayeb E M Eid, Zulkarnain Zainal, Mohd Saeed, Muna Ilowefah, Sharida Fakurazi, Norhaszalina Mohd Isa, Mohamed Ezzat El Zowalaty

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

In this study, in vitro cytotoxicity of nickel zinc (NiZn) ferrite nanoparticles against human colon cancer HT29, breast cancer MCF7, and liver cancer HepG2 cells was examined. The morphology, homogeneity, and elemental composition of NiZn ferrite nanoparticles were investigated by scanning electron microscopy, transmission electron microscopy, and energy dispersive X-ray spectroscopy, respectively. The exposure of cancer cells to NiZn ferrite nano-particles (15.6-1,000 μg/mL; 72 hours) has resulted in a dose-dependent inhibition of cell growth determined by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The quantification of caspase-3 and -9 activities and DNA fragmentation to assess the cell death pathway of the treated cells showed that both were stimulated when exposed to NiZn ferrite nanoparticles. Light microscopy examination of the cells exposed to NiZn ferrite nanoparticles demonstrated significant changes in cellular morphology. The HepG2 cells were most prone to apoptosis among the three cells lines examined, as the result of treatment with NiZn nanoparticles. In conclusion, NiZn ferrite nanoparticles are suggested to have potential cytotoxicity against cancer cells.

Original languageEnglish
Pages (from-to)2497-2508
Number of pages12
JournalInternational Journal of Nanomedicine
Volume8
DOIs
Publication statusPublished - 13 Jul 2013

Fingerprint

Cytotoxicity
Nanoparticles
Ferrite
Zinc
Epithelial Cells
Nickel
Cells
Neoplasms
Hep G2 Cells
Liver Neoplasms
Cell death
Breast Neoplasms
X-Ray Emission Spectrometry
Caspase 9
Cell growth
DNA Fragmentation
Transmission Electron Microscopy
Caspase 3
Liver
Electron Scanning Microscopy

Keywords

  • Anticancer
  • Apoptosis
  • Cancer cells lines
  • NiZn ferrite nanoparticles

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Organic Chemistry
  • Drug Discovery

Cite this

Al-Qubaisi, M. S., Rasedee, A., Flaifel, M. H., Ahmad, S., Hussein-Al-Ali, S., Hussein, M. Z., ... El Zowalaty, M. E. (2013). Cytotoxicity of nickel zinc ferrite nanoparticles on cancer cells of epithelial origin. International Journal of Nanomedicine, 8, 2497-2508. https://doi.org/10.2147/IJN.S42367

Cytotoxicity of nickel zinc ferrite nanoparticles on cancer cells of epithelial origin. / Al-Qubaisi, Mothanna Sadiq; Rasedee, Abdullah; Flaifel, Moayad Husein; Ahmad, Sahrim; Hussein-Al-Ali, Samer; Hussein, Mohd Zobir; Eid, Eltayeb E M; Zainal, Zulkarnain; Saeed, Mohd; Ilowefah, Muna; Fakurazi, Sharida; Mohd Isa, Norhaszalina; El Zowalaty, Mohamed Ezzat.

In: International Journal of Nanomedicine, Vol. 8, 13.07.2013, p. 2497-2508.

Research output: Contribution to journalArticle

Al-Qubaisi, MS, Rasedee, A, Flaifel, MH, Ahmad, S, Hussein-Al-Ali, S, Hussein, MZ, Eid, EEM, Zainal, Z, Saeed, M, Ilowefah, M, Fakurazi, S, Mohd Isa, N & El Zowalaty, ME 2013, 'Cytotoxicity of nickel zinc ferrite nanoparticles on cancer cells of epithelial origin', International Journal of Nanomedicine, vol. 8, pp. 2497-2508. https://doi.org/10.2147/IJN.S42367
Al-Qubaisi, Mothanna Sadiq ; Rasedee, Abdullah ; Flaifel, Moayad Husein ; Ahmad, Sahrim ; Hussein-Al-Ali, Samer ; Hussein, Mohd Zobir ; Eid, Eltayeb E M ; Zainal, Zulkarnain ; Saeed, Mohd ; Ilowefah, Muna ; Fakurazi, Sharida ; Mohd Isa, Norhaszalina ; El Zowalaty, Mohamed Ezzat. / Cytotoxicity of nickel zinc ferrite nanoparticles on cancer cells of epithelial origin. In: International Journal of Nanomedicine. 2013 ; Vol. 8. pp. 2497-2508.
@article{cb525a7c72ab4c808c48c2f9a43d8514,
title = "Cytotoxicity of nickel zinc ferrite nanoparticles on cancer cells of epithelial origin",
abstract = "In this study, in vitro cytotoxicity of nickel zinc (NiZn) ferrite nanoparticles against human colon cancer HT29, breast cancer MCF7, and liver cancer HepG2 cells was examined. The morphology, homogeneity, and elemental composition of NiZn ferrite nanoparticles were investigated by scanning electron microscopy, transmission electron microscopy, and energy dispersive X-ray spectroscopy, respectively. The exposure of cancer cells to NiZn ferrite nano-particles (15.6-1,000 μg/mL; 72 hours) has resulted in a dose-dependent inhibition of cell growth determined by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The quantification of caspase-3 and -9 activities and DNA fragmentation to assess the cell death pathway of the treated cells showed that both were stimulated when exposed to NiZn ferrite nanoparticles. Light microscopy examination of the cells exposed to NiZn ferrite nanoparticles demonstrated significant changes in cellular morphology. The HepG2 cells were most prone to apoptosis among the three cells lines examined, as the result of treatment with NiZn nanoparticles. In conclusion, NiZn ferrite nanoparticles are suggested to have potential cytotoxicity against cancer cells.",
keywords = "Anticancer, Apoptosis, Cancer cells lines, NiZn ferrite nanoparticles",
author = "Al-Qubaisi, {Mothanna Sadiq} and Abdullah Rasedee and Flaifel, {Moayad Husein} and Sahrim Ahmad and Samer Hussein-Al-Ali and Hussein, {Mohd Zobir} and Eid, {Eltayeb E M} and Zulkarnain Zainal and Mohd Saeed and Muna Ilowefah and Sharida Fakurazi and {Mohd Isa}, Norhaszalina and {El Zowalaty}, {Mohamed Ezzat}",
year = "2013",
month = "7",
day = "13",
doi = "10.2147/IJN.S42367",
language = "English",
volume = "8",
pages = "2497--2508",
journal = "International Journal of Nanomedicine",
issn = "1176-9114",
publisher = "Dove Medical Press Ltd.",

}

TY - JOUR

T1 - Cytotoxicity of nickel zinc ferrite nanoparticles on cancer cells of epithelial origin

AU - Al-Qubaisi, Mothanna Sadiq

AU - Rasedee, Abdullah

AU - Flaifel, Moayad Husein

AU - Ahmad, Sahrim

AU - Hussein-Al-Ali, Samer

AU - Hussein, Mohd Zobir

AU - Eid, Eltayeb E M

AU - Zainal, Zulkarnain

AU - Saeed, Mohd

AU - Ilowefah, Muna

AU - Fakurazi, Sharida

AU - Mohd Isa, Norhaszalina

AU - El Zowalaty, Mohamed Ezzat

PY - 2013/7/13

Y1 - 2013/7/13

N2 - In this study, in vitro cytotoxicity of nickel zinc (NiZn) ferrite nanoparticles against human colon cancer HT29, breast cancer MCF7, and liver cancer HepG2 cells was examined. The morphology, homogeneity, and elemental composition of NiZn ferrite nanoparticles were investigated by scanning electron microscopy, transmission electron microscopy, and energy dispersive X-ray spectroscopy, respectively. The exposure of cancer cells to NiZn ferrite nano-particles (15.6-1,000 μg/mL; 72 hours) has resulted in a dose-dependent inhibition of cell growth determined by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The quantification of caspase-3 and -9 activities and DNA fragmentation to assess the cell death pathway of the treated cells showed that both were stimulated when exposed to NiZn ferrite nanoparticles. Light microscopy examination of the cells exposed to NiZn ferrite nanoparticles demonstrated significant changes in cellular morphology. The HepG2 cells were most prone to apoptosis among the three cells lines examined, as the result of treatment with NiZn nanoparticles. In conclusion, NiZn ferrite nanoparticles are suggested to have potential cytotoxicity against cancer cells.

AB - In this study, in vitro cytotoxicity of nickel zinc (NiZn) ferrite nanoparticles against human colon cancer HT29, breast cancer MCF7, and liver cancer HepG2 cells was examined. The morphology, homogeneity, and elemental composition of NiZn ferrite nanoparticles were investigated by scanning electron microscopy, transmission electron microscopy, and energy dispersive X-ray spectroscopy, respectively. The exposure of cancer cells to NiZn ferrite nano-particles (15.6-1,000 μg/mL; 72 hours) has resulted in a dose-dependent inhibition of cell growth determined by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The quantification of caspase-3 and -9 activities and DNA fragmentation to assess the cell death pathway of the treated cells showed that both were stimulated when exposed to NiZn ferrite nanoparticles. Light microscopy examination of the cells exposed to NiZn ferrite nanoparticles demonstrated significant changes in cellular morphology. The HepG2 cells were most prone to apoptosis among the three cells lines examined, as the result of treatment with NiZn nanoparticles. In conclusion, NiZn ferrite nanoparticles are suggested to have potential cytotoxicity against cancer cells.

KW - Anticancer

KW - Apoptosis

KW - Cancer cells lines

KW - NiZn ferrite nanoparticles

UR - http://www.scopus.com/inward/record.url?scp=84880304249&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84880304249&partnerID=8YFLogxK

U2 - 10.2147/IJN.S42367

DO - 10.2147/IJN.S42367

M3 - Article

C2 - 23885175

AN - SCOPUS:84880304249

VL - 8

SP - 2497

EP - 2508

JO - International Journal of Nanomedicine

JF - International Journal of Nanomedicine

SN - 1176-9114

ER -