Cytotoxicity evaluation of Zingiber zerumbet ethyl acetate extract on K-562, erythroleukemia cell line

Asmah Hamid, Che Yang Aminah Ramly, Saiful Ridzuan Karim, Umi Noraashikin Zainudin, Nor Fadilah Rajab, Khairana Husain, Nurul Farhana Jufri

Research output: Contribution to journalArticle

Abstract

Background: Zingiber zerumbet locally known as lempoyang is used traditionally to cure swelling, sores, and loss of appetite. Its rhizomes contain many active compounds such as zerumbone and kaempferol that have been shown to exhibit antiproliferative activities on various cancer cell types. However, the effect of this plant on leukemia has not thoroughly been investigated. Objective: This study was conducted to investigate the cytotoxic effects of Z. zerumbet ethyl acetate crude extract on apoptosis-resistant erythroleukemia cell line, K-562. Materials and Methods: The cytotoxic effects and morphological changes of Z. zerumbet ethyl acetate crude extract on apoptosis-resistant erythroleukemia cell line, K-562, were investigated spectrometrically through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay and morphologically using the phase-contrast microscopy, respectively. Results: MTT assay demonstrated that this crude extract showed cytotoxic effects on erythroleukemia cell line K-562 in a dose-dependent and time-dependent manner with IC50values of 13.83 ± 1.82 and 10.13 ± 1.67 μg/ml for 48 and 72 h time points, respectively. The morphology of treated cells showed apparent changes of features such as membrane blebbing, cell shrinkage, and formation of apoptotic bodies which were the signs of apoptosis. The changes of cell morphology that resembled apoptotic features were more obvious with increase in treatment time. Conclusions: This finding indicates that ethyl acetate crude extract of Z. zerumbet exhibits cytotoxic effects on K-562 cell line through induction of apoptosis as morphologically seen in the cells. Further investigations are needed to ascertain the possibility of apoptosis as its major event of cell death.

Original languageEnglish
Pages (from-to)S430-S433
JournalPharmacognosy Magazine
Volume14
Issue number57
DOIs
Publication statusPublished - 1 Jul 2018

Fingerprint

Leukemia, Erythroblastic, Acute
Complex Mixtures
Apoptosis
Cell Line
Phase-Contrast Microscopy
Rhizome
Appetite
Blister
Leukemia
Cell Death
Cell Membrane
ethyl acetate
Neoplasms

Keywords

  • Crude extract
  • cytotoxic
  • erythroleukemia cell line K-562
  • morphological changes
  • Zingiber zerumbet

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Drug Discovery

Cite this

Cytotoxicity evaluation of Zingiber zerumbet ethyl acetate extract on K-562, erythroleukemia cell line. / Hamid, Asmah; Ramly, Che Yang Aminah; Karim, Saiful Ridzuan; Zainudin, Umi Noraashikin; Rajab, Nor Fadilah; Husain, Khairana; Jufri, Nurul Farhana.

In: Pharmacognosy Magazine, Vol. 14, No. 57, 01.07.2018, p. S430-S433.

Research output: Contribution to journalArticle

Hamid, Asmah ; Ramly, Che Yang Aminah ; Karim, Saiful Ridzuan ; Zainudin, Umi Noraashikin ; Rajab, Nor Fadilah ; Husain, Khairana ; Jufri, Nurul Farhana. / Cytotoxicity evaluation of Zingiber zerumbet ethyl acetate extract on K-562, erythroleukemia cell line. In: Pharmacognosy Magazine. 2018 ; Vol. 14, No. 57. pp. S430-S433.
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abstract = "Background: Zingiber zerumbet locally known as lempoyang is used traditionally to cure swelling, sores, and loss of appetite. Its rhizomes contain many active compounds such as zerumbone and kaempferol that have been shown to exhibit antiproliferative activities on various cancer cell types. However, the effect of this plant on leukemia has not thoroughly been investigated. Objective: This study was conducted to investigate the cytotoxic effects of Z. zerumbet ethyl acetate crude extract on apoptosis-resistant erythroleukemia cell line, K-562. Materials and Methods: The cytotoxic effects and morphological changes of Z. zerumbet ethyl acetate crude extract on apoptosis-resistant erythroleukemia cell line, K-562, were investigated spectrometrically through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay and morphologically using the phase-contrast microscopy, respectively. Results: MTT assay demonstrated that this crude extract showed cytotoxic effects on erythroleukemia cell line K-562 in a dose-dependent and time-dependent manner with IC50values of 13.83 ± 1.82 and 10.13 ± 1.67 μg/ml for 48 and 72 h time points, respectively. The morphology of treated cells showed apparent changes of features such as membrane blebbing, cell shrinkage, and formation of apoptotic bodies which were the signs of apoptosis. The changes of cell morphology that resembled apoptotic features were more obvious with increase in treatment time. Conclusions: This finding indicates that ethyl acetate crude extract of Z. zerumbet exhibits cytotoxic effects on K-562 cell line through induction of apoptosis as morphologically seen in the cells. Further investigations are needed to ascertain the possibility of apoptosis as its major event of cell death.",
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AU - Hamid, Asmah

AU - Ramly, Che Yang Aminah

AU - Karim, Saiful Ridzuan

AU - Zainudin, Umi Noraashikin

AU - Rajab, Nor Fadilah

AU - Husain, Khairana

AU - Jufri, Nurul Farhana

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AB - Background: Zingiber zerumbet locally known as lempoyang is used traditionally to cure swelling, sores, and loss of appetite. Its rhizomes contain many active compounds such as zerumbone and kaempferol that have been shown to exhibit antiproliferative activities on various cancer cell types. However, the effect of this plant on leukemia has not thoroughly been investigated. Objective: This study was conducted to investigate the cytotoxic effects of Z. zerumbet ethyl acetate crude extract on apoptosis-resistant erythroleukemia cell line, K-562. Materials and Methods: The cytotoxic effects and morphological changes of Z. zerumbet ethyl acetate crude extract on apoptosis-resistant erythroleukemia cell line, K-562, were investigated spectrometrically through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay and morphologically using the phase-contrast microscopy, respectively. Results: MTT assay demonstrated that this crude extract showed cytotoxic effects on erythroleukemia cell line K-562 in a dose-dependent and time-dependent manner with IC50values of 13.83 ± 1.82 and 10.13 ± 1.67 μg/ml for 48 and 72 h time points, respectively. The morphology of treated cells showed apparent changes of features such as membrane blebbing, cell shrinkage, and formation of apoptotic bodies which were the signs of apoptosis. The changes of cell morphology that resembled apoptotic features were more obvious with increase in treatment time. Conclusions: This finding indicates that ethyl acetate crude extract of Z. zerumbet exhibits cytotoxic effects on K-562 cell line through induction of apoptosis as morphologically seen in the cells. Further investigations are needed to ascertain the possibility of apoptosis as its major event of cell death.

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KW - morphological changes

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