Cytotoxicity assessment of organotin(IV) (2-metoxyethyl) methyldithiocarbamate compounds in human leukemia cell lines

Kamaludin Nurul Farahana, Siti Aishah Zakaria, Normah Awang, Rapidah Mohamad, Norraphat Uttraphan Pim

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Dibutyltin(IV) (2-methoxyethyl) methyldithiocarbamate (Compound 1) and tricyclohexyltin(IV) (2-methoxyethyl) methyldithiocarbamate (Compound 2) were used to evaluate their cytotoxicity against three human leukemic cell lines namely Jurkat E6.1, K562, and HL-60. The cytotoxicity of these compounds was assessed using MTT assay for treatment duration of 24 hours. The morphological changes were also observed upon the induction of these compounds with the IC50 value for 24 hours. Both compounds demonstrated high cytotoxicity towards the cell lines tested, with IC50 values of 0.14–1.30 µM (Jurkat E6.1), 0.06–0.18 µM (HL-60) and 5.20-5.40 µM (K562). The morphological changes of the cell lines induced by both compounds mostly showed characteristics of cell death via apoptosis. In conclusion, both compounds exhibited good cytotoxicity towards the cell lines tested. However, further studies are needed to identify the exact mode of cell death and the mechanisms of action of these compounds in induced human leukemic cell lines.

Original languageEnglish
Pages (from-to)1756-1766
Number of pages11
JournalOriental Journal of Chemistry
Volume33
Issue number4
DOIs
Publication statusPublished - 2017

Fingerprint

Cytotoxicity
Leukemia
Cells
Cell Line
Cell death
Inhibitory Concentration 50
Cell Death
apoptosis
assay
Assays
methyldithiocarbamate
cytotoxicity
leukaemia
Apoptosis

Keywords

  • Apoptosis
  • Cytotoxic
  • Dithiocarbamates
  • Leukemia
  • Organotin

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry
  • Environmental Chemistry
  • Drug Discovery

Cite this

Cytotoxicity assessment of organotin(IV) (2-metoxyethyl) methyldithiocarbamate compounds in human leukemia cell lines. / Nurul Farahana, Kamaludin; Zakaria, Siti Aishah; Awang, Normah; Mohamad, Rapidah; Pim, Norraphat Uttraphan.

In: Oriental Journal of Chemistry, Vol. 33, No. 4, 2017, p. 1756-1766.

Research output: Contribution to journalArticle

@article{51f1736c76974b79831d322e58a6acc4,
title = "Cytotoxicity assessment of organotin(IV) (2-metoxyethyl) methyldithiocarbamate compounds in human leukemia cell lines",
abstract = "Dibutyltin(IV) (2-methoxyethyl) methyldithiocarbamate (Compound 1) and tricyclohexyltin(IV) (2-methoxyethyl) methyldithiocarbamate (Compound 2) were used to evaluate their cytotoxicity against three human leukemic cell lines namely Jurkat E6.1, K562, and HL-60. The cytotoxicity of these compounds was assessed using MTT assay for treatment duration of 24 hours. The morphological changes were also observed upon the induction of these compounds with the IC50 value for 24 hours. Both compounds demonstrated high cytotoxicity towards the cell lines tested, with IC50 values of 0.14–1.30 µM (Jurkat E6.1), 0.06–0.18 µM (HL-60) and 5.20-5.40 µM (K562). The morphological changes of the cell lines induced by both compounds mostly showed characteristics of cell death via apoptosis. In conclusion, both compounds exhibited good cytotoxicity towards the cell lines tested. However, further studies are needed to identify the exact mode of cell death and the mechanisms of action of these compounds in induced human leukemic cell lines.",
keywords = "Apoptosis, Cytotoxic, Dithiocarbamates, Leukemia, Organotin",
author = "{Nurul Farahana}, Kamaludin and Zakaria, {Siti Aishah} and Normah Awang and Rapidah Mohamad and Pim, {Norraphat Uttraphan}",
year = "2017",
doi = "10.13005/ojc/330420",
language = "English",
volume = "33",
pages = "1756--1766",
journal = "Oriental Journal of Chemistry",
issn = "0970-020X",
publisher = "Bhopal, S.A. Iqbal",
number = "4",

}

TY - JOUR

T1 - Cytotoxicity assessment of organotin(IV) (2-metoxyethyl) methyldithiocarbamate compounds in human leukemia cell lines

AU - Nurul Farahana, Kamaludin

AU - Zakaria, Siti Aishah

AU - Awang, Normah

AU - Mohamad, Rapidah

AU - Pim, Norraphat Uttraphan

PY - 2017

Y1 - 2017

N2 - Dibutyltin(IV) (2-methoxyethyl) methyldithiocarbamate (Compound 1) and tricyclohexyltin(IV) (2-methoxyethyl) methyldithiocarbamate (Compound 2) were used to evaluate their cytotoxicity against three human leukemic cell lines namely Jurkat E6.1, K562, and HL-60. The cytotoxicity of these compounds was assessed using MTT assay for treatment duration of 24 hours. The morphological changes were also observed upon the induction of these compounds with the IC50 value for 24 hours. Both compounds demonstrated high cytotoxicity towards the cell lines tested, with IC50 values of 0.14–1.30 µM (Jurkat E6.1), 0.06–0.18 µM (HL-60) and 5.20-5.40 µM (K562). The morphological changes of the cell lines induced by both compounds mostly showed characteristics of cell death via apoptosis. In conclusion, both compounds exhibited good cytotoxicity towards the cell lines tested. However, further studies are needed to identify the exact mode of cell death and the mechanisms of action of these compounds in induced human leukemic cell lines.

AB - Dibutyltin(IV) (2-methoxyethyl) methyldithiocarbamate (Compound 1) and tricyclohexyltin(IV) (2-methoxyethyl) methyldithiocarbamate (Compound 2) were used to evaluate their cytotoxicity against three human leukemic cell lines namely Jurkat E6.1, K562, and HL-60. The cytotoxicity of these compounds was assessed using MTT assay for treatment duration of 24 hours. The morphological changes were also observed upon the induction of these compounds with the IC50 value for 24 hours. Both compounds demonstrated high cytotoxicity towards the cell lines tested, with IC50 values of 0.14–1.30 µM (Jurkat E6.1), 0.06–0.18 µM (HL-60) and 5.20-5.40 µM (K562). The morphological changes of the cell lines induced by both compounds mostly showed characteristics of cell death via apoptosis. In conclusion, both compounds exhibited good cytotoxicity towards the cell lines tested. However, further studies are needed to identify the exact mode of cell death and the mechanisms of action of these compounds in induced human leukemic cell lines.

KW - Apoptosis

KW - Cytotoxic

KW - Dithiocarbamates

KW - Leukemia

KW - Organotin

UR - http://www.scopus.com/inward/record.url?scp=85031946176&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85031946176&partnerID=8YFLogxK

U2 - 10.13005/ojc/330420

DO - 10.13005/ojc/330420

M3 - Article

AN - SCOPUS:85031946176

VL - 33

SP - 1756

EP - 1766

JO - Oriental Journal of Chemistry

JF - Oriental Journal of Chemistry

SN - 0970-020X

IS - 4

ER -