Cytotoxicity and mode of cell death induced by triphenyltin (IV) compounds in vitro

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Abstract

A series of newly synthesized organotin (IV) with N-alkyl-N-phenyldithiocarbamate ligands namely triphenyltin (IV) ethylphenyldithiocarbamate (compound 1) and triphenyltin (IV) butylphenyldithiocarbamate (compound 2) were assessed for their cytotoxic effect against HT-29 human colon adenocarcinoma cells and human CCD-18Co normal colon cells. The cytotoxicity of these organotins in both cells was assessed using 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazholium bromide (MTT) assay upon 24 h treatment. Both compounds demonstrated potent cytotoxicity towards HT-29 cells with the IC50 of 0.18 μM for compound 1 and 0.20 μM for compound 2. Interestingly, compound 1 exhibited lower cytotoxicity towards CCD-18Co with IC50 of 1.55 μM whereas no IC50 was detected for compound 2 up to 2 μM treatment. The mode of cell death was determined based on the externalization of phosphatidylserine using flow cytometry. Cells treated with compound 1 and compound 2 were mainly viable and the apoptotic cell death was around 10% which suggests that both compounds induced growth arrest. In conclusion, this study demonstrated that both compounds were selective towards human colorectal cells by giving a strong cytotoxicity to cancer cells and low toxicity towards normal cells. Both compounds were suggested to induce growth arrest in HT-29 cells.

Original languageEnglish
Pages (from-to)84-93
Number of pages10
JournalOnLine Journal of Biological Sciences
Volume14
Issue number2
DOIs
Publication statusPublished - 2014

Fingerprint

Cell death
Cytotoxicity
cytotoxicity
cell death
Cell Death
Charge coupled devices
Inhibitory Concentration 50
HT29 Cells
cells
inhibitory concentration 50
Flow cytometry
Phosphatidylserines
Bromides
Colon
colon
Toxicity
Assays
Cells
Ligands
phosphatidylserines

Keywords

  • Apoptosis
  • CCD-18Co
  • Cytotoxicity
  • Dithiocarbamate
  • HT-29
  • Organotin (IV)

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

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title = "Cytotoxicity and mode of cell death induced by triphenyltin (IV) compounds in vitro",
abstract = "A series of newly synthesized organotin (IV) with N-alkyl-N-phenyldithiocarbamate ligands namely triphenyltin (IV) ethylphenyldithiocarbamate (compound 1) and triphenyltin (IV) butylphenyldithiocarbamate (compound 2) were assessed for their cytotoxic effect against HT-29 human colon adenocarcinoma cells and human CCD-18Co normal colon cells. The cytotoxicity of these organotins in both cells was assessed using 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazholium bromide (MTT) assay upon 24 h treatment. Both compounds demonstrated potent cytotoxicity towards HT-29 cells with the IC50 of 0.18 μM for compound 1 and 0.20 μM for compound 2. Interestingly, compound 1 exhibited lower cytotoxicity towards CCD-18Co with IC50 of 1.55 μM whereas no IC50 was detected for compound 2 up to 2 μM treatment. The mode of cell death was determined based on the externalization of phosphatidylserine using flow cytometry. Cells treated with compound 1 and compound 2 were mainly viable and the apoptotic cell death was around 10{\%} which suggests that both compounds induced growth arrest. In conclusion, this study demonstrated that both compounds were selective towards human colorectal cells by giving a strong cytotoxicity to cancer cells and low toxicity towards normal cells. Both compounds were suggested to induce growth arrest in HT-29 cells.",
keywords = "Apoptosis, CCD-18Co, Cytotoxicity, Dithiocarbamate, HT-29, Organotin (IV)",
author = "Normah Awang and Aziz, {Zalila Abdul} and {Nurul Farahana}, Kamaludin and {Kok Meng}, Chan",
year = "2014",
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TY - JOUR

T1 - Cytotoxicity and mode of cell death induced by triphenyltin (IV) compounds in vitro

AU - Awang, Normah

AU - Aziz, Zalila Abdul

AU - Nurul Farahana, Kamaludin

AU - Kok Meng, Chan

PY - 2014

Y1 - 2014

N2 - A series of newly synthesized organotin (IV) with N-alkyl-N-phenyldithiocarbamate ligands namely triphenyltin (IV) ethylphenyldithiocarbamate (compound 1) and triphenyltin (IV) butylphenyldithiocarbamate (compound 2) were assessed for their cytotoxic effect against HT-29 human colon adenocarcinoma cells and human CCD-18Co normal colon cells. The cytotoxicity of these organotins in both cells was assessed using 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazholium bromide (MTT) assay upon 24 h treatment. Both compounds demonstrated potent cytotoxicity towards HT-29 cells with the IC50 of 0.18 μM for compound 1 and 0.20 μM for compound 2. Interestingly, compound 1 exhibited lower cytotoxicity towards CCD-18Co with IC50 of 1.55 μM whereas no IC50 was detected for compound 2 up to 2 μM treatment. The mode of cell death was determined based on the externalization of phosphatidylserine using flow cytometry. Cells treated with compound 1 and compound 2 were mainly viable and the apoptotic cell death was around 10% which suggests that both compounds induced growth arrest. In conclusion, this study demonstrated that both compounds were selective towards human colorectal cells by giving a strong cytotoxicity to cancer cells and low toxicity towards normal cells. Both compounds were suggested to induce growth arrest in HT-29 cells.

AB - A series of newly synthesized organotin (IV) with N-alkyl-N-phenyldithiocarbamate ligands namely triphenyltin (IV) ethylphenyldithiocarbamate (compound 1) and triphenyltin (IV) butylphenyldithiocarbamate (compound 2) were assessed for their cytotoxic effect against HT-29 human colon adenocarcinoma cells and human CCD-18Co normal colon cells. The cytotoxicity of these organotins in both cells was assessed using 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazholium bromide (MTT) assay upon 24 h treatment. Both compounds demonstrated potent cytotoxicity towards HT-29 cells with the IC50 of 0.18 μM for compound 1 and 0.20 μM for compound 2. Interestingly, compound 1 exhibited lower cytotoxicity towards CCD-18Co with IC50 of 1.55 μM whereas no IC50 was detected for compound 2 up to 2 μM treatment. The mode of cell death was determined based on the externalization of phosphatidylserine using flow cytometry. Cells treated with compound 1 and compound 2 were mainly viable and the apoptotic cell death was around 10% which suggests that both compounds induced growth arrest. In conclusion, this study demonstrated that both compounds were selective towards human colorectal cells by giving a strong cytotoxicity to cancer cells and low toxicity towards normal cells. Both compounds were suggested to induce growth arrest in HT-29 cells.

KW - Apoptosis

KW - CCD-18Co

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KW - Organotin (IV)

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