Cytotoxicity activity of biotransformed ethyl p-methoxycinnamate by Aspergillus niger

Muhammad Nor Omar, Nor Hazwani Mohd Hasali, Mohd. Ambar Yarmo

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The extraction of Kaempferia galanga rhizome using steam distillation and supercritical fluid extraction (SFE) was carried out. After fractionation, the major compound of the K. galanga extract, ethyl p-methoxycinnamate (EPMC) was transformed using Aspergillus niger into ethyl p-hydroxycinnamate (EPHC). The biological anticancer activity of EPMC and its biotransformed product (EPHC) was established by cytotoxicity activity on the human breast cancer (MCF-7) cell line using MTT assay. Ethyl p-hydroxycinnamate (EPHC) was most cytotoxic against MCF-7 at 1000 μg/mL where percentage of cell viability was 9.87 %, while IC50 was 340 μg/mL. EPHC showed slightly higher cytotoxicity activity compared to EPMC. The results of this study show that the biotransformation process was able to produce a metabolite (EPHC) with higher cytotoxicity activity compared to its parent compound (EPMC).

Original languageEnglish
Pages (from-to)2731-2734
Number of pages4
JournalOriental Journal of Chemistry
Volume32
Issue number5
DOIs
Publication statusPublished - 2016

Fingerprint

Aspergillus niger
Aspergillus
Cytotoxicity
Alpinia
Cells
Zingiberaceae
biotransformation
rhizome
Supercritical Fluid Chromatography
distillation
Rhizome
Distillation
Supercritical fluids
cancer
metabolite
MCF-7 Cells
Steam
viability
Fractionation
fractionation

Keywords

  • Biotransformation
  • Cytotoxic
  • Ethyl p-methoxycinnamate
  • Kaempferia galanga

ASJC Scopus subject areas

  • Chemistry(all)
  • Environmental Chemistry
  • Biochemistry
  • Drug Discovery

Cite this

Cytotoxicity activity of biotransformed ethyl p-methoxycinnamate by Aspergillus niger. / Omar, Muhammad Nor; Hasali, Nor Hazwani Mohd; Yarmo, Mohd. Ambar.

In: Oriental Journal of Chemistry, Vol. 32, No. 5, 2016, p. 2731-2734.

Research output: Contribution to journalArticle

Omar, Muhammad Nor ; Hasali, Nor Hazwani Mohd ; Yarmo, Mohd. Ambar. / Cytotoxicity activity of biotransformed ethyl p-methoxycinnamate by Aspergillus niger. In: Oriental Journal of Chemistry. 2016 ; Vol. 32, No. 5. pp. 2731-2734.
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