Cytotoxic effect of red seaweeds kappaphycus alvarezii and kappaphycus striatum on hepatocarcinoma HepG2 cell line

Farah Diyana Ariffin, Aminah Abdullah, Chan Kok Meng, Shahrul Hisham Zainal Ariffin, Mazrura Sahani

Research output: Contribution to journalArticle

Abstract

Background: Food antioxidants have been considered as effective agents to reduce oxidative stress which can lead to cancer.

Objective: The aim of this study was to investigate the potential cytotoxic effect of antioxidant extracts of two commonly found seaweeds namely Kappaphycus alvarezii and Kappaphycus striatum against hepatocarcinoma HepG2 cell.

Methods: Cell viability was evaluated by the 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Annexin V-FITC/PI flowcytometry assay was used to determine the cell death mode of HepG2 cells treated by K. alvarezii and K. striatum extracts.

Results: The IC50 concentration of K. alvarezii and K. striatum extracts that inhibit the proliferation of hepatocarcinoma HepG2 cells was 1.8 mg/mL and 0.9 mg/mL respectively. This finding showed that the antioxidant extracts of K. striatum exhibited better antiproliferative effect against hepatocarcinoma HepG2 cell than the antioxidant extracts of K. alvarezii. However, using Annexin V-FITC/PI showed more than 80% of hepatocarcinoma HepG2 cell were viable after treatment with IC50 concentration of each K. alvarezii and K. striatum extract. This result suggested that cytostatic effect of K. alvarezii and K. striatum extracts to hepatocarcinoma HepG2 cells was found at high concentrations.

Conclusion: The result of the study indicated that antioxidant extracts of K. alvarezii and K. striatum did not show cytotoxic effect to hepatocarcinoma HepG2 cells.

Original languageEnglish
Pages (from-to)79-84
Number of pages6
JournalAdvances in Environmental Biology
Volume8
Issue number15
Publication statusPublished - 1 Jan 2014

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Kappaphycus
Kappaphycus alvarezii
Seaweed
Hep G2 Cells
seaweed
antioxidant
macroalgae
cytotoxicity
cell lines
Cell Line
Antioxidants
extracts
antioxidants
Fluorescein-5-isothiocyanate
Annexin A5
cells
assay
Inhibitory Concentration 50
inhibitory concentration 50
bromide

ASJC Scopus subject areas

  • Environmental Science(all)
  • Agricultural and Biological Sciences(all)

Cite this

@article{260910f756854e2a90796d86dc7d8816,
title = "Cytotoxic effect of red seaweeds kappaphycus alvarezii and kappaphycus striatum on hepatocarcinoma HepG2 cell line",
abstract = "Background: Food antioxidants have been considered as effective agents to reduce oxidative stress which can lead to cancer.Objective: The aim of this study was to investigate the potential cytotoxic effect of antioxidant extracts of two commonly found seaweeds namely Kappaphycus alvarezii and Kappaphycus striatum against hepatocarcinoma HepG2 cell.Methods: Cell viability was evaluated by the 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Annexin V-FITC/PI flowcytometry assay was used to determine the cell death mode of HepG2 cells treated by K. alvarezii and K. striatum extracts.Results: The IC50 concentration of K. alvarezii and K. striatum extracts that inhibit the proliferation of hepatocarcinoma HepG2 cells was 1.8 mg/mL and 0.9 mg/mL respectively. This finding showed that the antioxidant extracts of K. striatum exhibited better antiproliferative effect against hepatocarcinoma HepG2 cell than the antioxidant extracts of K. alvarezii. However, using Annexin V-FITC/PI showed more than 80{\%} of hepatocarcinoma HepG2 cell were viable after treatment with IC50 concentration of each K. alvarezii and K. striatum extract. This result suggested that cytostatic effect of K. alvarezii and K. striatum extracts to hepatocarcinoma HepG2 cells was found at high concentrations.Conclusion: The result of the study indicated that antioxidant extracts of K. alvarezii and K. striatum did not show cytotoxic effect to hepatocarcinoma HepG2 cells.",
author = "Ariffin, {Farah Diyana} and Aminah Abdullah and {Kok Meng}, Chan and {Zainal Ariffin}, {Shahrul Hisham} and Mazrura Sahani",
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T1 - Cytotoxic effect of red seaweeds kappaphycus alvarezii and kappaphycus striatum on hepatocarcinoma HepG2 cell line

AU - Ariffin, Farah Diyana

AU - Abdullah, Aminah

AU - Kok Meng, Chan

AU - Zainal Ariffin, Shahrul Hisham

AU - Sahani, Mazrura

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Background: Food antioxidants have been considered as effective agents to reduce oxidative stress which can lead to cancer.Objective: The aim of this study was to investigate the potential cytotoxic effect of antioxidant extracts of two commonly found seaweeds namely Kappaphycus alvarezii and Kappaphycus striatum against hepatocarcinoma HepG2 cell.Methods: Cell viability was evaluated by the 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Annexin V-FITC/PI flowcytometry assay was used to determine the cell death mode of HepG2 cells treated by K. alvarezii and K. striatum extracts.Results: The IC50 concentration of K. alvarezii and K. striatum extracts that inhibit the proliferation of hepatocarcinoma HepG2 cells was 1.8 mg/mL and 0.9 mg/mL respectively. This finding showed that the antioxidant extracts of K. striatum exhibited better antiproliferative effect against hepatocarcinoma HepG2 cell than the antioxidant extracts of K. alvarezii. However, using Annexin V-FITC/PI showed more than 80% of hepatocarcinoma HepG2 cell were viable after treatment with IC50 concentration of each K. alvarezii and K. striatum extract. This result suggested that cytostatic effect of K. alvarezii and K. striatum extracts to hepatocarcinoma HepG2 cells was found at high concentrations.Conclusion: The result of the study indicated that antioxidant extracts of K. alvarezii and K. striatum did not show cytotoxic effect to hepatocarcinoma HepG2 cells.

AB - Background: Food antioxidants have been considered as effective agents to reduce oxidative stress which can lead to cancer.Objective: The aim of this study was to investigate the potential cytotoxic effect of antioxidant extracts of two commonly found seaweeds namely Kappaphycus alvarezii and Kappaphycus striatum against hepatocarcinoma HepG2 cell.Methods: Cell viability was evaluated by the 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Annexin V-FITC/PI flowcytometry assay was used to determine the cell death mode of HepG2 cells treated by K. alvarezii and K. striatum extracts.Results: The IC50 concentration of K. alvarezii and K. striatum extracts that inhibit the proliferation of hepatocarcinoma HepG2 cells was 1.8 mg/mL and 0.9 mg/mL respectively. This finding showed that the antioxidant extracts of K. striatum exhibited better antiproliferative effect against hepatocarcinoma HepG2 cell than the antioxidant extracts of K. alvarezii. However, using Annexin V-FITC/PI showed more than 80% of hepatocarcinoma HepG2 cell were viable after treatment with IC50 concentration of each K. alvarezii and K. striatum extract. This result suggested that cytostatic effect of K. alvarezii and K. striatum extracts to hepatocarcinoma HepG2 cells was found at high concentrations.Conclusion: The result of the study indicated that antioxidant extracts of K. alvarezii and K. striatum did not show cytotoxic effect to hepatocarcinoma HepG2 cells.

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