Kesan sitotoksik dan aktiviti anti-MRSA ekstrak metanol phyllanthus gracilipes dan phyllanthus columnaris

Translated title of the contribution: Cytotoxic effect and anti-MRSA activity of methanolic extracts of phyllanthus gracilipes and phyllanthus columnaris

G. M. Fazari, A. Azilawaty, Nazlina Ibrahim, Wan Yaacob Wan Ahmad

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A study was carried out to test the cytotoxic effect and antibacterial activity of methanol extracts from different parts of Phyllanthus gracilipes and P. columnaris. The extraction was done at room temperature and using a Soxhlet apparatus to produce 22 extracts. Phytochemical test on the extracts produced using Soxhlet showed the presence of saponins in all the extracts whilst steroids were found in all P. gracilipes parts and only in 3 extracts of P. columnaris. Cytotoxic tests on Vero cell culture revealed four extracts were cytotoxic with LC 50 values below 20 μg/m L i.e. room temperature extract of P. gracilipes stem-bark, Soxhlet extracts of P. gracilipes stem-wood and leaves, and room temperature extract of P. columnaris root-wood. Room temperature and Soxhlet extracts of P. gracilipes stem-wood, and all room temperature extracts of P. columnaris inhibited the growth of 13 isolates of MRSA (Methicillin-Resistant Staphylococcus aureus) tested.

Original languageUndefined/Unknown
Pages (from-to)457-466
Number of pages10
JournalSains Malaysiana
Volume40
Issue number5
Publication statusPublished - May 2011

Fingerprint

Phyllanthus
cytotoxicity
extracts
ambient temperature
stemwood
methicillin-resistant Staphylococcus aureus
testing
saponins
lethal concentration 50
phytopharmaceuticals
steroids
bark
cell culture
methanol

Keywords

  • Antibacterial activity
  • Cytotoxicity
  • Phyllanthus columnaris
  • Phyllanthus gracilipes
  • Vera cell

ASJC Scopus subject areas

  • General

Cite this

Kesan sitotoksik dan aktiviti anti-MRSA ekstrak metanol phyllanthus gracilipes dan phyllanthus columnaris. / Fazari, G. M.; Azilawaty, A.; Ibrahim, Nazlina; Wan Ahmad, Wan Yaacob.

In: Sains Malaysiana, Vol. 40, No. 5, 05.2011, p. 457-466.

Research output: Contribution to journalArticle

Fazari, G. M. ; Azilawaty, A. ; Ibrahim, Nazlina ; Wan Ahmad, Wan Yaacob. / Kesan sitotoksik dan aktiviti anti-MRSA ekstrak metanol phyllanthus gracilipes dan phyllanthus columnaris. In: Sains Malaysiana. 2011 ; Vol. 40, No. 5. pp. 457-466.
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